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  • 1
    Electronic Resource
    Electronic Resource
    An improved perfusion fixation method for the testis (1977)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 188 (1977), S. 307-314 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: A reliable and uniform vascular perfusion fixation method for the testis has been developed by using an initial washout solution containing a vasodilator and an anticoagulant. This is followed by a brief fixation with a sodium phosphate buffered formaldehyde-glutaraldehyde solution of conventional strength, and then a second more concentrated aldehyde fixative solution containing picric acid. The method takes into account some of the unique features of the vascular supply of the male genital tract for its favorable perfusion and fixation. The advantages of this method are: (1) consistently favorable preservation of the testis; (2) simple and inexpensive apparatus; and (3) stable and relatively innocuous stock solutions.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091880304
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  • 2
    Electronic Resource
    Electronic Resource
    Staining of stratified squamous epithelium of mucous membranes and skin of man and monkey by the periodic acid-Schiff methodThe work was aided by grants from the American Cancer Society: (1) on the recommendation of the Committee on Growth of the National Research Council, and (2) from an Institutional Grant to Harvard University. (1951)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 110 (1951), S. 359-375 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091100307
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Unusual features of the nuclear envelope in human spermatogenic cellsSupported by Research Grants HD 02344 and HD 06969 and by a Public Health Service International Research Fellowship 5-FO5-TWO 2223-02 (Dr. H. Chemes). (1978)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 192 (1978), S. 493-511 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Different types of human germ cells show unusual features of the nuclear envelope. Spermatogonial nuclei demonstrate two kinds of modifications. The first one is a series of intranuclear flattened cisterns, parallel to each other and to the inner aspect of the nuclear envelope. The second one is a nuclear envelope protrusion into the cytoplasm occupied by a double membrane-limited vesicle. Pores are found on the membrane of the vesicle facing the interior of the nucleus. In spermatocytes the nuclear pores are concentrated over certain areas and completely absent from others. In the regions where they are absent a single cytoplasmic cistern of rough endoplasmic reticulum is closely apposed to the outer membrane of the nuclear envelope. Early modifications of the nuclear surface appear in spermatids before the attachment of the acrosomic vesicle and may indicate an active role of the nuclear envelope in the morphogenesis of the acrosome. In round spermatids nuclear pores are absent from the area which is first related to the Golgi and later covered by the acrosomal cap. Single or multiple layers of cytoplasmic annulate lamellae are closely associated with the nuclear envelope over the pore rich areas. Frequently there are intranuclear accumulations of dense material adjacent to the annulate lamellae-nuclear pore complex. The chromatoid body is usually present on the cytoplasmic side of this complex. In the elongating spermatids most annulate lamellae are free in the cytoplasm, often in relation with Golgi and chromatoid body remnants near the axial filament. Few stacks of annulate la-mellae are noted adjacent to the pore rich nuclear regions. It is suggested that the described modifications are related to an active nuclear-cytoplasmic interaction.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091920404
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  • 4
    Electronic Resource
    Electronic Resource
    A reexamination of motility patterns of rat epididymal spermatozoaSupported in part by contract NIH-69-2107 from the Center of Population Studies of the National Institute of Child Health and Human Developmentand by grant 710-0036 from the Ford Foundation. (1972)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 173 (1972), S. 301-307 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The swimming motion of rat epididymal spermatozoa was reexamined using high speed cinematography. Sperm were filmed at 100 frames per second and viewed at a reduced speed for detailed study. The flagella of spermatozoa from the caput epididymidis exhibit a rigid curved middle piece proximal to the cytoplasmic droplet and a flexible portion distal to the droplet. Bending waves begin at the caudal end of the immobile middle piece and propagate distally. The large majority of sperm from this region of the epididymis swim in a circular fashion, clockwise or counterclockwise, depending on the orientation of the curved midpiece. A few spermatozoa, however, manage to achieve a form of forward progression by alternation of the direction of curvature of their inflexible anterior segment. Spermatozoa from the cauda epididymidis swim progressively forward and rotate frequently about their axis of progression. Propagated waves of bending arise in the neck region and involve the midpiece as well as the more distal segments of the tail. The changing pattern of motility of sperm as they mature in the epididymis is consistent with the hypothesis, suggested by others, that the components of motility may develop independently and sequentially and further suggests that the site of initiation of bending waves is not limited to the centriole.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091730305
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  • 5
    Electronic Resource
    Electronic Resource
    Sertoli cells of the golden-mantled ground squirrel (Spermophilus lateralis): A model system for the study of shape change (1983)
    New York, NY [u.a.] : Wiley-Blackwell
    American Journal of Anatomy 168 (1983), S. 83-98 
    Details
    ISSN: 0002-9106
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Sertoli cells of the ground squirrel (Spermophilus lateralis), a seasonal breeder, were examined by light and electron microscopy and their structure, particularly the organization of the cytoskeleton, was related to events that occur in the seminiferous epithelium during spermatogenesis. Among the events considered and described are the apical movement of elongate spermatids, withdrawal of residual cytoplasm from germ cells, transport of smooth endoplasmic reticulum (SER) between the base and apex of the Sertoli cells, and sperm release. These events are dramatically evident in this species because the seminiferous epithelium is thin, i.e., there are few germ cells, and both the germ cells and Sertoli cells are large.Sertoli cells of the ground squirrel have a remarkably well developed cytoskeleton. Microfilaments occur throughout the cell but are most evident in ectoplasmic specializations associated with junctions. Intermediate filaments occur around the nucleus, as a layer at the base of the cell, and adjacent to desmosome-like junctions with germ cells. Intermediate filaments, together with microtubules, are also abundant in regions of the cell involved with the transport of SER, in cytoplasm associated with elongate spermatids, and in processes that extend into the residual cytoplasm of germ cells. Our observations of ultrastructure are consistent with the hypothesis that Sertoli cell microtubules are involved with the movement of germ cells within the seminiferous epithelium, and further implicate these structures as possibly playing a role in the retraction of residual cytoplasm from germ cells and the intracellular transport of SER. The abundance and organization of intermediate filaments suggest that these cytoskeletal elements may also be involved with events that occur during spermatogenesis.
    Additional Material: 12 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aja.1001680109
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  • 6
    Electronic Resource
    Electronic Resource
    Morphology of the epithelium of the extratesticular rete testis, ductuli efferentes and ductus epididymidis of the adult male rabbitResearch supported by USPHS Grants HD-04290 (DWH) and HD-02344 (DWF), by the Ford Foundation (DWF) and by a Milton Fund grant from Harvard University (DWH). (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    American Journal of Anatomy 156 (1979), S. 373-400 
    Details
    ISSN: 0002-9106
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The fine structure of the epithelium lining the extratesticular rete testis, ductuli efferentes and ductus epididymidis of the rabbit has been investigated. In the ductuli efferentes the epithelium is composed of two cell types, principal cells and ciliated cells. The latter cell type is distinguished from principal cells by the presence of cilia projecting into the lumen and the position of the nucleus in the apical half of the cell. Principal cells in this segment are characterized by micropinocytotic vesicles on the surface plasma membrane and a variety of small dense bodies scattered throughout the cytoplasm. In the ductus epididymidis basal cells replace ciliated cells as the second cell type, but differences between various segments of the epididymis are related to the fine structure of the principal cells. In the proximal caput epididymidis (Nicander's region 1) the principal cells are tall with long microvilli. They typically contain a small Golgi apparatus and a cluster of dense bodies adjacent to the nucleus. In the distal caput epididymidis (Nicander's regions 2-5) the apical cytoplasm of principal cells is filled with numerous micropinocytotic vesicles and large multivesicular bodies; these features are interpreted as signs of absorptive activity. The multivesicular bodies are absent from the cytoplasm of principal cells in the corpus epididymidis (Nicander's region 6) and, instead, numerous elements of smooth endoplasmic reticulum, a large Golgi apparatus, lipid droplets and dense bodies characterize principal cells in this segment. Towards the proximal cauda epididymidis (Nicander's region 7), the number of dense bodies (lysosomes) in the cytoplasm increases considerably. In the globose cauda (Nicander's region 8), the principal cells are reduced in height, and in addition to the features described in region 7, are characterized by a concentric array of rough endoplasmic reticulum in the basal cytoplasm. These observations are discussed in relation to the role of the epididymis in promoting the maturation and survival of spermatozoa.
    Additional Material: 28 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aja.1001560307
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