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In situ activated T lymphocytes in active versus stable periodontal lesions (1988)

Reinhardt, R. A. ; McDonald, T. L. ; Bolton, R. W. ; [et al.]

Oxford, UK : Blackwell Publishing Ltd

Journal of periodontal research 23 (1988), S. 0

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ISSN: 1600-0765

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Medicine

Notes: A double labeling technique was developed to detect T cell phenotype and HLA-DR (activation) antigens on infiltrating cells in gingival biopsy sites classified as either periodontally active (≥ mm attachment loss within past 3 months), clinically similar but stable, or healthy. Serial cryostat sections were obtained from each of the above disease category biopsies in 13 periodontal maintenance patients, and were processed with sequential Leu series (T cell subsets) avidinbiotin-peroxidase followed by anti-HLA-DR indirect alkaline phosphatase labeling protocols. Labeled cells were counted in repeatable fields in the sulcular, middle and oral thirds of each section, and HLA-DR+ T cells (activated) were calculated. Total HLA-DR-labeled cells and HLA-DR-positive pan-T cells and T helper cells (Th) were all more numerous in active specimens than in healthy sites (p〈0.05), and in sulcular fields than in the oral third of the section (p〈0.05). Although activated T suppressor cell (T5) densities did not vary statistically according to tissue location, active biopsies had more cells per field than either stable or healthy specimens (p〈0.05), especially in the sulcular third. A majority of pan-T, Ts and Th cells appeared to display activation markers in active, stable and healthy biopsies. This study supports the functional role of T lymphocytes in modulating the immune response in periodontal tissues.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1111/j.1600-0765.1988.tb01420.x

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Alkaline and acid phosphatases from the extensively halotolerant bacteriumHalomonas elongata (1990)

Bylund, J. E. ; Dyer, J. K. ; Feely, D. E. ; [et al.]

Springer

Current microbiology 20 (1990), S. 125-131

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ISSN: 1432-0991

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Medicine

Notes: Abstract Alkaline and acid phosphatases (EC 3.1.3.1 and EC 3.1.3.2, respectively) ofHalomonas elongata were cytochemically localized on the cell envelope. These enzymes were then isolated and partially purified by sonication, ammonium sulfate precipitation, and column chromatography from cells grown in alanine defined medium at 0.05, 1.37, and 3.4M NaCl. Enzyme assays were conducted at pH 5.0 and 9.0 with varying concentrations of NaCl, KCl, and LiCl in the assay buffer. Results showed higher acid phosphatase activity compared with that of alkaline phosphatase; and all enzyme activities were optimal at NaCl concentrations similar to the medium NaCl concentrations for the cells grown at 1.37 and 3.4M. However, minimum enzyme activities were observed for cells grown at the low salt concentration (0.05M). Although samples showed strong activities at some KCl concentrations, generally the enzyme activities decreased significantly when KCl or LiCl was substituted for NaCl. Polyacrylamide gel electrophoresis followed by histochemical staining for the phosphatases showed only one band for both enzymes for each cell sample grown at the different NaCl concentrations.