ISSN:
1432-0983
Keywords:
Sheep
;
Mitochondrial DNA
;
Physical mapping
;
Gene localization
;
Cloning
;
Digoxigenin
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Mitochondrial DNA from the liver of a single Rasa Aragonesa sheep has been isolated and characterized. The size of the genome, determined by restriction enzyme analysis, was found to be 16.58 kbp. The cleavage sites for the restriction endonucleases BamHI, HindIII, EcoRI, BglII, PvuII, BstEII and PstI were mapped, and the gene organization deduced through heterologous hybridization using different cloned fragments of the rat mitochondrial genome. Fragments representative of the entire sheep genome were cloned in plasmid vectors pGEM3Z and pUN121.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00336847
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