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  • 1
    Electronic Resource
    Electronic Resource
    Sequence-specific binding by Aspergillus nidulans AflR, a C6 zinc cluster protein regulating mycotoxin biosynthesis (1998)
    Oxford BSL : Blackwell Science Ltd
    Molecular microbiology 28 (1998), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The Aspergillus nidulans aflR gene is found within a 60 kb gene cluster that includes ≈24 other genes that putatively function in the production of the aflatoxin-related mycotoxin sterigmatocystin. Previous work showed that AflR is a C6 zinc binuclear cluster protein that is conserved across Aspergillus spp. and functions as a pathway-specific transcription factor in activating expression of other cluster genes. In this report, we demonstrate that A. nidulans AflR (AnAflR) is a 45 kDa protein that binds to the palindromic sequence 5′-TCG(N5)CGA-3′ found in the promoter regions of several aflatoxin and sterigmatocystin cluster genes (stc genes). The in vivo relevance of this AnAflR binding site was assessed by examining the contribution of the three TCG(N5)CGA elements in the 1.1 kb promoter region of stcU using gene fusions with the bacterial uidA gene encoding β-glucuronidase (GUS). By mutating one, two or all three of the AnAflR-binding elements and examining GUS activity in wild-type aflR or ΔaflR A. nidulans strains, we found that stc gene activation required both AnAflR and at least one TCG(N5)CGA AflR binding site.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2958.1998.00907.x
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  • 2
    Electronic Resource
    Electronic Resource
    Conservation of structure and function of the aflatoxin regulatory gene aflR from Aspergillus nidulans and A. flavus (1996)
    Springer
    Current genetics 29 (1996), S. 549-555 
    Details
    ISSN: 1432-0983
    Keywords: Key words aflR ; Aflatoxin ; Secondary metabolism ; Zinc binuclear cluster
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Under limiting growth conditions, Aspergillus nidulans produces a carcinogenic secondary metabolite related to aflatoxin and called sterigmatocystin (ST). The genes for ST biosynthesis are co-ordinately regulated and are all found within an approximately 60-kilobase segment of DNA. One of the genes within this region is predicted to encode a CX2CX6CX6CX2CX6CX2 zinc binuclear cluster DNA-binding protein that is related to the Aspergillus flavus and Aspergillus parasiticus aflatoxin regulatory gene aflR. Deletion of the A. nidulans aflR homolog resulted in an inability to induce expression of genes within the ST gene cluster and a loss of ST production. Because A. nidulans aflR mRNA accumulates specifically under conditions that favor ST production we expect that activation of ST biosynthetic genes is determined by A. nidulans aflR. In support of this hypothesis, we demonstrated that induced expression of the A. flavus aflR gene in A. nidulans, under conditions that normally suppress ST gene expression, resulted in activation of genes in the ST biosynthetic pathway. This result demonstrates that AflR function is conserved between Aspergillus spp. and that aflR expression is sufficient to activate genes in the ST pathway.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002940050084
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Conservation of structure and function of the aflatoxin regulatory geneaflR fromAspergillus nidulans andA. flavus (1996)
    Springer
    Current genetics 29 (1996), S. 549-555 
    Details
    ISSN: 1432-0983
    Keywords: allR ; Aflatoxin ; Secondary metabolism ; Zinc binuclear cluster
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Under limiting growth conditions,Aspergillus nidulans produces a carcinogenic secondary metabolite related to aflatoxin and called sterigmatocystin (ST). The genes for ST biosynthesis are co-ordinately regulated and are all found within an approximately 60-kilobase segment of DNA. One of the genes within this region is predicted to encode a CX2CX6CX6CX2CX6CX2 zinc binuclear cluster DNA-binding protein that is related to theAspergillus flavus andAspergillus parasiticus aflatoxin regulatory geneaflR. Deletion of theA. nidulans aflR homolog resulted in an inability to induce expression of genes within the ST gene cluster and a loss of ST production. BecauseA. nidulans aflR mRNA accumulates specifically under conditions that favor ST production we expect that activation of ST biosynthetic genes is determined byA. nidulans aflR. In support of this hypothesis, we demonstrated that induced expression of theA. flavus aflR gene inA. nidulans, under conditions that normally suppress ST gene expression, resulted in activation of genes in the ST biosynthetic pathway. This result demonstrates that AflR function is conserved betweenAspergillus spp. and thataflR expression is sufficient to activate genes in the ST pathway.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02426959
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    Paper (German National Licenses)
    Fulltext
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