ISSN:
1573-6784
Source:
Springer Online Journal Archives 1860-2000
Topics:
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Summary The transformation of Phaffia rhodozyma by electroporation was better than the one by the lithium chloride (LiCl) or spheroplast transformation methods. The influence of several parameters on transformation efficiency was studied. Electroporation conditions of 1200 Volts, 50 μFarads and 2000 Ohms were proved successful. A maximum of 1.5 × 103 transformants per 100 ng of DNA was reached.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00180396
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