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  • 1
    Electronic Resource
    Electronic Resource
    High prevalence of IgE antibodies among blood donors in Sweden and Norway (2005)
    Oxford, UK : Munksgaard International Publishers
    Allergy 60 (2005), S. 0 
    Details
    ISSN: 1398-9995
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background:  Reactions after a blood transfusion could be allergic because of passive transfer of immunoglobulin E (IgE) antibodies from allergic donors.Aims of the study:  To compare spectrum and prevalence of IgE antibodies in blood donors from Sweden and Norway.Methods:  Using the ImmunoCAP method, serum samples from 1002 blood donors from Sweden and 500 from Norway were analysed for IgE antibodies to common inhalant and food allergens and allergens common in a hospital environment, such as penicilloyl G and latex.Results:  As many as 23.6–27.3% of the donors had IgE antibodies to at least one of the 14 allergens tested. Of these 6.8–16.7% had extremely high concentrations, i.e. 〉35 kUA/l corresponding to 100 times the cut-off for a positive allergy test. Most donors were sensitized to pollens, dander and mite but several had very high levels of IgE antibodies to penicilloyl G, latex and peanut. The pattern of sensitizing allergens differed between Sweden and Norway.Conclusions:  High serum levels of IgE antibodies to various allergens are common among blood donors and the degree of sensitization and spectrum of involved allergen varies between geographical regions. Present routines to identify IgE sensitized, potential risk, donors are not satisfactory; the sensitivity of selection procedures is about 25%.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1398-9995.2005.00896.x
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  • 2
    Electronic Resource
    Electronic Resource
    Comparative Studies on Tree Pollen Allergens (1987)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 26 (1987), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Visualization by autoradiography of specific IgE binding in crossed radioimmunoelectrophoresis (CRIE) and other 125I-immunoautoradiography (IAR) techniques is done in two different ways; either by traditional direct autoradiography (D-ARG), where the film is exposed to the 125I-anti-lgE incubated sample at room temperature, or by indirect autoradiography (ID-ARG), applying intensifying screen, low-temperature exposure and, eventually, pre-exposure. This study confirmed that D-ARG provided the benefits of simplicity and better image resolution with the disadvantage of prolonged exposure periods. ID-ARG reduced the exposures needed to produce film image densities of 0.01 and 0.1 A540 nm (i.e. autoradiographic sensitivity (ARσ) and autoradiographic speed (ARs)) to 1/l8 and 1/55 respectively of the corresponding exposures in D-ARG. The lowest detection limits for 125I in 24 h were 1.2 cpm mm−2 with the indirect and 6.8cpm mm−2 with the direct systems investigated. The major drawbacks of ID-ARG were inferior image resolution and higher background levels, especially when pre-exposure was included.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1987.tb02275.x
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  • 3
    Electronic Resource
    Electronic Resource
    Allergenic Synthetic Peptide Corresponding to the Second Calcium-Binding Loop of Cod Allergen M (1981)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 14 (1981), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: A peptidewith the sequence of the second calcium-binding loop (EF loop) of cod Allergen M was synthesized by automatic solid-phase technique. The synthetic peptide corresponded to residues 88–103 of the known primary structure of Allergen M. The immunochemical reactivity of this loop, previously demonstrated for the overlapping enzymic fragments, was confirmed by using the synthetic preparation. The purified hexadecapeptide was shown to bind specifically to reaginic IgE from sera of cod-allergic individuals, in both in vivo and in vitro test systems. It could also bind rabbit anti-Allergen M, as shown by rocket line immunoelectrophoresis and quantitative precipitation inhibition techniques. The findings emphasized that the immunological reactivity of the synthetic peptide (88–103) was compatible with a monovalent haptenic function: blocking and not eliciting allergic reactions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1981.tb00201.x
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  • 4
    Electronic Resource
    Electronic Resource
    Plasmapheresis in the treatment of steroid-dependent bronchial asthma (2001)
    Copenhagen : Munksgaard International Publishers
    Allergy 56 (2001), S. 0 
    Details
    ISSN: 1398-9995
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background: Can plasmapheresis improve disease severity and lung function and reduce steroid doses in severe asthma patients dependent on oral corticosteroids? Methods: A pilot study with four asthma patients was undertaken using PEF (peak expiratory flow) symptom score, number of puffs of β2-agonist, and dose of systemic steroids as disease variables. After at least an 8-week run-in, the patients were randomized to a crossover treatment regimen consisting of either 10 days of plasmapheresis or placebo treatment. Each treatment was succeeded by an 8–26-week follow-up period. Results: No patients achieved a significant effect of plasmapheresis treatment according to the established criteria, nor did anyone experience deterioration. Conclusions: Removing humoral factors extensively over a 10-day period did not significantly influence the clinical condition of the four steroid-dependent asthma patients studied. Circulating humoral factors seem to be of little importance for the maintenance of the established chronic allergic inflammation in these patients. Plasmapheresis does not seem to be a treatment option for this patient category.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1034/j.1398-9995.2001.00141.x
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  • 5
    Electronic Resource
    Electronic Resource
    The Immunological Reactivity of the Three Homologous Repetitive Tetrapeptides in the Region 41–64 of Allergen M from Cod (1982)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 16 (1982), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The CD-calcium binding loop of Allergen M encompasses the two homologous tetrapep-tides Asp-Glu-Asp-Lys and Asp-Glu-Leu-Lys. These tetrapeptides were recently suggested to be mutually critical for the immunological specificity of this region. To obtain evidence of the validity of this hypothesis, four peptide analogues encompassing these tetrapeptides at the termini were produced by manual solid-phase peptide synthesis. The structures of these peptides were ascertained by amino acid analyses and end-group sequence determination. The crude preparations were prepared by high-performance liquid chromatography and high-voltage electrophoresis. The immunological reactivity was investigated by using the preliminary purified peptides in the IgE and IgG test systems used. The results of radioallergosorbent test inhibition showed that all the synthetic peptides encompassing a minimum of two of the tetrapeptides have the capacity to bind specific IgE of the serum pool. The composition and sequence of the six spacer amino acids between these tetrapeptides were of no importance for the acitivity. The highest inhibition percentage was obtained by the peptides derived from the primary structure of Allergen M. No activity was found for the control peptides synthesized under identical conditions. Similar results were shown by PIC tests, using two cod-allergic sera and two recipients. In the IgG test system, all the four peptides containing the two terminal tetrapeptides showed an antigenic reactivity. They deflected allergen M line in rocket line immunoelectrophoresis. No deflection was given by the control peptides or proteins used. We conclude that the immunological reactivity of region 41–64 of allergen M is determined by 3 homologous tetrapeptides, repeated in three sites interspaced by six amino acids in a segment of 24 residues.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1982.tb00701.x
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