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  • 1
    Electronic Resource
    Electronic Resource
    Wide mapping of a T-DNA insertion site in oilseed rape using bulk segregant analysis and comparative mapping (1997)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 116 (1997), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Doubled haploid oilseed rape lines segregating for a transgene inducing herbicide resistance (bar gene) were investigated for the wide mapping of the T-DNA insertion site. Bulk segregant analysis using presence/absence and intensity polymorphisms between the bulks, as well as comparative mapping with a linkage group deriving from another cross, led to the identification of 11 random amplified polymorphic DNA (RAPD) markers tightly or loosely linked to the bar gene. Ten RAPD loci out of 11 were located on the same side of the bar locus, strongly suggesting that the T-DNA integrated in a telomeric or subtelomeric position. The eleventh RAPD marker exhibited a strong segregation distortion, which could be the result of a heteroduplex formation. Comparison of the linkage groups obtained from the two crosses showed different recombination rates between markers, possibly reflecting differences in parental genetic backgrounds. Consequences and potential applications in transgene dispersal safety assessment studies are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0523.1997.tb02189.x
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  • 2
    Electronic Resource
    Electronic Resource
    Segregation Analysis of Isozyme Markers on Isolated Microspore-Derived Embryos in Brassica napus L. (1993)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 110 (1993), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The segregation of isozyme genetic markers was studied on embryos arising from isolated microspore cultures in live rapeseed F1 hybrid genotypes. Out of the ten isozyme genes considered, live (Aco-1, Aco-3, Lp-1, Pgm-3, Tpi-1) did not segregate according to expected Mendelian ratios in at least two F1 crosses. F2 plants, generated from the same hybrids, included in the analysis, were tree of segregation distortions. Linkage analysis between each segregating enzyme locus in each progeny revealed independance between these markers.In this paper, the results of the linkage analysis as well as the origins of the distortions are discussed. The existence of androgenic embryogenesis genes may be responsible for the abnormal segregations of the five isozyme genes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0523.1993.tb00595.x
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    In vitro androgenesis and segregation distortion in Brassica napus L.: spontaneous versus colchicine-doubled lines (1997)
    Springer
    Plant cell reports 16 (1997), S. 464-468 
    Details
    ISSN: 1432-203X
    Keywords: Key words Brassicaceae ; Microspore cultures ; Haploid plants ; Molecular markers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A total of 750 plantlets were regenerated from 1,400 embryos produced through microspore cultures from one F1 plant of the cross `Darmor-bzh' × `Yudal'. Fifty-three percent of the regenerants were evaluated by flow cytometric analysis, which revealed that 31% were spontaneous diploid (SD), 63% were still haploid and the remaining 6% plants had other ploidy levels. Available segregation data (266 markers) produced on this androgenic progeny were used to study the interference between segregation distortion and the mode of chromosome doubling of androgenc lines. On the basis of the present results it is not possible to conclude that distortions are peculiar to one type of regenerated plant; only a difference in the intensity of the bias might be assumed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01092767
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    In vitro androgenesis and segregation distortion inBrassica napus L.: spontaneous versus colchicine-doubled lines (1997)
    Springer
    Plant cell reports 16 (1997), S. 464-468 
    Details
    ISSN: 1432-203X
    Keywords: Brassicaceae ; Microspore cultures ; Haploid plants ; Molecular markers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A total of 750 plantlets were regenerated from 1,400 embryos produced through microspore cultures from one F1 plant of the cross ‘Darmor-bzh’ x ‘Yudal’. Fifty-three percent of the regenerants were evaluated by flow cytometric analysis, which revealed that 31% were spontaneous diploid (SD), 63% were still haploid and the remaining 6% plants had other ploidy levels. Available segregation data (266 markers) produced on this androgenic progeny were used to study the interference between segregation distortion and the mode of chromosome doubling of androgenc lines. On the basis of the present results it is not possible to conclude that distortions are peculiar to one type of regenerated plant; only a difference in the intensity of the bias might be assumed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01092767
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Molecular-mapping analysis in Brassica napus using isozyme, RAPD and RFLP markers on a doubled-haploid progeny (1996)
    Springer
    Theoretical and applied genetics 93 (1996), S. 1017-1025 
    Details
    ISSN: 1432-2242
    Keywords: Brassica napus ; L. Genetic mapping ; RFLP and RAPD markers ; Segregation distortions ; Comparative mapping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have undertaken the construction of a Brassica napus genetic map with isozyme (4%), RFLP (26.5%) and RAPD (68%) markers on a 152 lines of a doubled-haploid population. The map covers 1765 cM and comprises 254 markers including three PCR-specific markers and a morphological marker. They are assembled into 19 linkage groups, covering approximatively 71% of the rapeseed genome. Thirty five percent of the studied markers did not segregate according to the expected Mendelian ratio and tended to cluster in eight specific linkage groups. In this paper, the structure of the genetic map is described and the existence of non-Mendelian segregations in linkage analysis as well as the origins of the observed distortions, are discussed. The mapped RFLP loci corresponded to the cDNAs already used to construct B. napus maps. The first results of intraspecific comparative mapping are presented.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00230119
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  • 6
    Electronic Resource
    Electronic Resource
    Molecular-mapping analysis in Brassica napus using isozyme, RAPD and RFLP markers on a doubled-haploid progeny (1996)
    Springer
    Theoretical and applied genetics 93 (1996), S. 1017-1025 
    Details
    ISSN: 1432-2242
    Keywords: Key wordsBrassica napus ; L. Genetic mapping ; RFLP and RAPD markers ; Segregation distortions ; Comparative mapping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have undertaken the construction of a Brassica napus genetic map with isozyme (4%), RFLP (26.5%) and RAPD (68%) markers on a 152 lines of a doubled-haploid population. The map covers 1765 cM and comprises 254 markers including three PCR-specific markers and a morphological marker. They are assembled into 19 linkage groups, covering approximatively 71% of the rapeseed genome. Thirty five percent of the studied markers did not segregate according to the expected Mendelian ratio and tended to cluster in eight specific linkage groups. In this paper, the structure of the genetic map is described and the existence of non-Mendelian segregations in linkage analysis as well as the origins of the observed distortions, are discussed. The mapped RFLP loci corresponded to the cDNAs already used to construct B. napus maps. The first results of intraspecific comparative mapping are presented.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050329
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    Paper (German National Licenses)
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  • 7
    Electronic Resource
    Electronic Resource
    Identification of loci contributing to quantitative field resistance to blackleg disease, causal agent Leptosphaeria maculans (Desm.) Ces. et de Not., in Winter rapeseed (Brassica napus L.) (1998)
    Springer
    Theoretical and applied genetics 96 (1998), S. 23-30 
    Details
    ISSN: 1432-2242
    Keywords: Key words Leptosphaeria maculans ; Brassica napus L ; Quantitative resistance ; QTL mapping ; Doubled-haploid progeny
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Blackleg, caused by Leptosphaeria maculans, is one of the most important diseases of Brassica napus. Genomic regions controlling blackleg resistance at the adult plant stage were detected using 152 doubled-haploid (DH) lines derived from the F1‘Darmor-bzh’בYudal’. The rapeseed genetic map used includes 288 DNA markers on 19 linkage groups. Blackleg resistance of each DH line was evaluated in field tests in 1995 and 1996 by measuring the mean disease index (I) and the percentage of lost plants (P). From notations recovered in 1995, ten quantitative trait loci (QTL) were detected: seven QTL for I and six QTL for P, explaining 57% and 41% of the genotypic variation, respectively. Three of them were common to I and P. From data recovered in 1996, seven QTL were identified: five QTL for I and two different QTL for P, accounting for 50% and 23% of the genotypic variation, respectively. One I QTL, located close to a dwarf gene (bzh), was detected with a very strong effect, masking more QTL detection. It was not revealed at the same position and with the same effect in 1995. Four major genomic regions were revealed from 1995 and from 1996 with the same parental contribution. One of them, located on the DY2 group, has a resistance allele from the susceptible parent. Five- and two-year-specific QTL were detected in 1995 and 1996, respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050704
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  • 8
    Electronic Resource
    Electronic Resource
    Characterisation of the radish introgression carrying the Rfo restorer gene for the Ogu-INRA cytoplasmic male sterility in rapeseed (Brassica napus L.) (1998)
    Springer
    Theoretical and applied genetics 97 (1998), S. 129-134 
    Details
    ISSN: 1432-2242
    Keywords: Key words Brassica napus ; Raphanus sativus ; Restorer gene ; Introgression ; RFLP ; RAPD ; Genetic mapping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Bulked segregant analysis and comparative mapping were applied to identify molecular markers linked to the Rfo restorer gene used for the Ogu-INRA cytoplasmic male-sterility system in rapeseed. These markers were then used to localise the radish introgression on the B. napus genetic map constructed from the cross ‘Darmor.bzh’ x ’Yudal’. The introgression mapped on the DY15 linkage group. From the comparison of this latter group to the linkage group constructed on a F2 progeny segregating for the radish introgression, it was concluded that the introgression had occurred through homoeologous recombination, that it was not distal and that it had replaced a B. napus region of around 50 cM. A QTL involved in aliphatic seed glucosinolate content was located on the DY15 linkage group at a position corresponding to one end of the introgression. The DNA markers identified in this study are being used in map-based cloning of the Rfo gene and in marker-assisted selection.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050876
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    Paper (German National Licenses)
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  • 9
    Electronic Resource
    Electronic Resource
    Identification of QTL involved in field resistance to light leaf spot (Pyrenopeziza brassicae) and blackleg resistance (Leptosphaeria maculans) in winter rapeseed (Brassica napus L.) (1998)
    Springer
    Theoretical and applied genetics 97 (1998), S. 398-406 
    Details
    ISSN: 1432-2242
    Keywords: Key words Pyrenopeziza brassicae ; Leptosphaeria maculans ; Brassica napus L. ; QTL mapping ; Colocalizations
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Quantitative trait loci (QTL), involved in the polygenic field resistance of rapeseed (Brassica napus L.) to light leaf spot disease, were mapped using 288 DNA markers on 152 doubled-haploid (DH) lines derived from the cross ‘Darmor-bzh’בYudal’. Over two years (1995 and 1996), the DH population was evaluated for light leaf spot resistance on leaves (L) and stems (S), and for blackleg disease resistance in same field trials. For the L resistance criterion, a total of five and seven QTL were detected in 1995 and in 1996 respectively, accounting for 53% and 57% of the genotypic variation. For the S criterion, three and five QTL were identified in 1995 and in 1996 respectively, explaining 29% and 43% of the genotypic variation. The locations of the QTL detected were quite consistent over the two years (4- and 2-year common QTL for L and S, respectively). Three genomic regions, located on the DY5, DY10 and DY11 groups, were common to the resistance on leaves and stems. In comparison with the QTL for blackleg resistance described by Pilet et al. (1998), two regions on the DY6 and DY10 groups, were associated with the two disease resistances. These ‘multiple disease resistance’ (‘MDR’) QTL may correspond to genes involved in common resistance mechanisms towards the two pathogens or else to clusters of resistance genes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050909
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    Paper (German National Licenses)
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  • 10
    Electronic Resource
    Electronic Resource
    Development of a SCAR (sequence characterised amplified region) marker for molecular tagging of the dwarf BREIZH (Bzh) gene in Brassica napus L. (1998)
    Springer
    Theoretical and applied genetics 97 (1998), S. 828-833 
    Details
    ISSN: 1432-2242
    Keywords: Key words: Brassica napus ; Dwarfing gene ; PCR markers ; Marker-assisted selection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  A SCAR (sequence characterised amplified region) has been developed for optimal tagging of the dwarf Bzh gene in Brassica napus. A RAPD marker named OPMO7-730 was previously found closely linked to the dwarf locus at 0.8±0.7 cM. The DNA band corresponding to this marker was cloned and sequenced, and specific PCR primers were designed. The PCR test allowed us to amplify the locus corresponding to OPM07-730. With a restriction endonuclease digest and optimal electrophoresis conditions, three allelic forms of this marker have been recovered on the 40 B. napus accessions tested. The usefullness of this marker in breeding dwarf rapeseed lines is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050962
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