Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Factors influencing the incidence of habituation for cytokinin of tobacco pith tissue in culture (1980)
    Springer
    Planta 150 (1980), S. 264-268 
    Details
    ISSN: 1432-2048
    Keywords: Cytokinin habituation ; Habituation, cytokinin ; Nicotiana ; Position effects (habituation) ; Size effects (habituation) ; Tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Pith tissue of Nicotiana tabacum L. cv. “Havana 425” exhibits a gradient in its tendency to habituate for cytokinin on an auxin-containing medium at 35° C, about 10° C above the standard culture temperature. Explants of pith from below the 8th to 11th internode, counting from the bottom of the plant, rarely habituate for cytokinin; explants from above this threshold habituate rapidly. The explants must also be above a critical size, about 20–30 mg, to habituate. There was a pronounced interaction between size and position effects; the threshold position for cytokinin habituation shifted upward with decreasing explant size.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00390836
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Quantitative studies of bud initiation in cultured tobacco tissues (1982)
    Springer
    Planta 155 (1982), S. 473-477 
    Details
    ISSN: 1432-2048
    Keywords: Auxin ; Bud initiation ; Competence ; Cytokinin ; Nicotiana (regeneration) ; Tissue culture (position effects)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract After transferring leaf, pith, and stemcortex tissues ofNicotiana tabacum L. cv. “Havana 425” from a complete medium containing auxin and cytokinin to an inductive medium with auxin deleted, there is lag phase of approx. 14d followed by a linear phase in which the rate of bud initiation is constant. The incidence of buds formed is very low, approx. one bud per 103 or 104 cells. Statistical analysis of the distribution of buds among explants and subcloning experiments provide evidence that the paucity of buds results from neither negative interactions among bud forming centers nor a paucity of cells with the potential for organogenesis. Our results are consistent with the hypothesis that the frequency of bud initiation is determined by the availability of competent cells, by position effects, or by a combination of both mechanisms.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01607570
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Evidence for a Mendelian factor controlling the cytokinin requirement of cultured tobacco cells (1983)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 4 (1983), S. 129-141 
    Details
    ISSN: 0192-253X
    Keywords: cytokin mutant ; habituation ; Nicotiana ; tissue culture ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Cultured leaf tissues of Nicotiana tabacum L. cv. “Havana 425” normally require an exogenous source of cytokinin for rapid growth; stem-cortex tissues do not - ie, they exhibit the cytokinin-habituated phenotype. We found that plants regenerated from cloned cortex and leaf tissues from one particular plant differed in leaf-tissue phenotype: Leaf tissues derived from leaf cells exhibited the normal, nonhabituated phenotype, whereas leaf tissues derived from cortex cells were cytokinin-habituated. This difference in leaf phenotype was not found using leaf and cortex cells from six other donor plants. The inheritance of the habituated leaf trait was studied in tissues from cortex-derived plants and hybrids between these plants and normal plants. F1 hybrids were intermediate between the parental types in degree of habituation. No differences were found between reciprocal hybrids. These results suggest that the habituated leaf trait is an incompletely dominant, nuclear trait. Both parental and intermediate phenotypes were recovered in the F2 progeny. The frequency of habituated leaf progeny in the F2 and backcross populations provide evidence that the trait is regulated at a single genetic locus.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020040207
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    A cytokinin mutant derived from cultured tobacco cells (1986)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 7 (1986), S. 159-165 
    Details
    ISSN: 0192-253X
    Keywords: cytokinin mutant ; habituated-leaf trait ; Nicotiana ; tissue culture ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Tissues cultured from the leaf lamina of wild-type Nicotiana tabacum L. cv. “Havana 425” plants require an exogenous source of cytokinin for rapid growth. In contrast, leaf tissues of plants heterozygous or homozygous for the partially dominant, monogenic habituated leaf (H1-1) trait, exhibit a cytokinin-autotrophic phenotype in culture. Here we show that the H1 trait can arise in culture. Cytokinin autotrophic variants were obtained by culturing wild-type tissues of leaf lamina successively on media containing reduced concentrations of the cytokinin, kinetin. Plants regenerated from clones of these variants exhibited the H1 phenotype, which segregated in breeding tests as expected for a dominant, monogenic trait. This trait, designated H1-2, is inherited at a different locus than the H1-1 trait described earlier. Our results show that cytokinin mutants can arise in cell culture and that at least two genes regulate the cytokinin requirement of cultured tobacco tissues.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020070305
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...