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  • 1
    Electronic Resource
    Electronic Resource
    Genetic transformation of green bean callus via Agrobacterium mediated DNA transfer (1993)
    Springer
    Plant cell reports 12 (1993), S. 74-79 
    Details
    ISSN: 1432-203X
    Keywords: Green bean ; Phaseolus vulgaris L ; Genetic transformation ; Stable integration ; chalcone synthase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Kanamycin resistant callus was produced from leaf disc or hypocotyl expiants of green bean (Phaseolus vulgaris L.) when cultured on a defined medium containing 50 mg/l kanamycin after 4 days of co-cultivation with Agrobacterium tumefaciens strain EHA101 containing the binary vector pKYLX71GUS. The presence of neomycin phosphotransferase II (NPT-II) in crude cellular extracts from the kanamycin resistant callus was confirmed by ELISA. The expression of the ß-glucuronidase (GUS) reporter gene was confirmed by histochemical and fluorimetric analyses. Southern blot border analysis confirmed the integration of the foreign DNA. In addition to the evidence obtained from Southern analysis, the absence of Agrobacterium in the transformed callus cultures was confirmed by microscopic observation and through test cultures. Using the above protocol, bean callus cultures were also transformed with a bean chalcone synthase promoter-GUS fusion. These cultures, when treated with the elicitor glutathione, showed higher levels of GUS expression than the unelicited callus clumps.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00241938
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Plant regeneration from seedling explants of green bean (Phaseolus vulgaris L) via organogenesis (1991)
    Springer
    Plant cell, tissue and organ culture 24 (1991), S. 199-206 
    Details
    ISSN: 1573-5044
    Keywords: cotyledon ; green bean ; meristematic ring ; Phaseolus vulgaris ; regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Green bean (Phaseolus vulgaris L.) plants were regenerated from 3-day old seedling explants via organogenesis. The explants contained a cotyledon and a small portion (2–3 mm) of embryonic axis split in half. Explants were cultured on a defined medium containing glutamine as the sole nitrogen source. A ring of meristematic tissue was produced at the base of the axillary bud located at the cotyledonary node. The meristematic tissue was produced only if the axillary bud was present together with the cotyledon in the explant. Buds and shoots developed from the meristematic ring. Selected shoots produced roots when excised from the cluster of buds and transferred to root induction medium. Rooted shoots (plantlets) grew well and produced viable seeds when grown in the greenhouse. Histological studies revealed the origin of buds from the peripheral layers of the meristematic ring. Production of buds and shoots was a continuous process, so that new shoots could be removed from the explant for plantlet production every 10–14 days. With the cultivar Dark Red Kidney, an average of 49 buds and 8 shoots were regenerated per explant by 30 days after culture initiation. Sixty-seven percent of the shoots produced roots, and 90–95% of the plantlets survived greenhouse acclimatization to produce healthy plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00033477
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Stable transformation of peanut callus viaAgrobacterium-mediated DNA transfer (1993)
    Springer
    Transgenic research 2 (1993), S. 321-324 
    Details
    ISSN: 1573-9368
    Keywords: Peanut ; Arachis hypogaea ; transformation ; callus ; Agrobacterium tumefaciens ; peanut stripe virus coat protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Transformed callus was produced from peanut (Arachis hypogaea L. cv. Okrun) hypocotyl explants after four days of co-cultivation withAgrobacterium tumefaciens strains EHA101, LBA4404 or ASE1 carrying the binary vector pKYLX71GUS on a defined medium followed by selection with kanamycin (200 mg l−1). Transformed calluses were cultured as independent cell lines potentially derived from a single transformation event. Stable integration and expression of foreign gene(s) in the callus was confirmed by Southern and western blot analyses and enzyme assays. A few cell lines showed a single insert of the foreign gene. Using the above protocol, transformed peanut callus expressing the peanut stripe virus coat protein gene was obtained.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01976172
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    Paper (German National Licenses)
    Fulltext
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