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  • 1
    ISSN: 1432-0789
    Keywords: Dehydrogenase activity ; Soil microbial activity ; Triphenyltetrazolium chloride (TTC) ; Iodonitrotetrazolium chloride (INT) ; Incubation conditions ; Extraction procedure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The triphenyltetrazolium chloride (TTC) method described by Thalmann (1968) and the iodonitrotetrazolium chloride (INT) method described by Spothelfer-Magaña and Thalmann (1992), used for measuring soil dehydrogenase activity, have been modified to overcome some methodical short-comings. Absorption maxima of 485 nm for triphenylformazan dissolved in acetone, 491 nm for iodonitrotetrazolium formazan (INTF) dissolved in tetrahydrofuran and 455 nm for INTF dissolved in N,N-dimethylformamide are recommended for measuring wavelengths. Extracting triphenylformazan twice with acetone is less toxic and proved to be at least as efficient as extraction with a mixture of 90% acetone and 10% carbon tetrachloride (Thalmann 1968 method). Tetrahydrofuran and dimethylformamide were equally good in extracting INTF from soils, but the former was less toxic. Anaerobic incubation resulted in the formation of higher amounts of triphenylformazan and INTF as well as reduced standard error. Both TTC and INT reduction showed high reproducibility and good differentiation of the microbial activity of six soils. For several reasons (more easily determined substrate dose depending on different soil types, better reduction, shorter incubation time), INT reduction seems to be a more suitable method of measuring soil microbial activity than TTC reduction.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-0789
    Keywords: Key words Waste water irrigation ; Heavy metals ; Soil organic matter ; Microbial biomass ; Microbial activity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract  The effect of long-term waste water irrigation (up to 80 years) on soil organic matter, soil microbial biomass and its activities was studied in two agricultural soils (Vertisols and Leptosols) irrigated for 25, 65 and 80 years respectively at Irrigation District 03 in the Valley of Mezquital near Mexico City. In the Vertisols, where larger amounts of water have been applied than in the Leptosols, total organic C (TOC) contents increased 2.5-fold after 80 years of irrigation. In the Leptosols, however, the degradability of the organic matter tended to increase with irrigation time. It appears that soil organic matter accumulation was not due to pollutants nor did microbial biomass:TOC ratios and qCO2 values indicate a pollutant effect. Increases in soil microbial biomass C and activities were presumably due to the larger application of organic matter. However, changes in soil microbial communities occurred, as denitrification capacities increased greatly and adenylate energy charge (AEC) ratios were reduced after long-term irrigation. These changes were supposed to be due to the addition of surfactants, especially alkylbenzene sulfonates (effect on denitrification capacity) and the addition of sodium and salts (effect on AEC) through waste water irrigation. Heavy metals contained in the sewage do not appear to be affecting soil processes yet, due to their low availability. Detrimental effects on soil microbial communities can be expected, however, from further increases in pollutant concentrations due to prolonged application of untreated waste water or an increase in mobility due to higher mineralization rates.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Biology and fertility of soils 28 (1999), S. 397-402 
    ISSN: 1432-0789
    Keywords: Key words Surfactants ; Alkylbenzene sulfonate ; Denitrification ; Soil microbial activity ; Wastewater irrigation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract  In semi-arid regions wastewater irrigation is a valuable resource for agricultural production. The contamination of irrigated soils with surfactants is one of the ecological risks related to irrigating with untreated wastewater. In this study, the effects of branched alkylbenzene sulfonates (ABS) on microbial biomass, respiratory activity, and denitrification capacity of soil samples (eutric vertisols) taken from an irrigation district in the Mexico City area were investigated in laboratory experiments. Increasing concentrations of ABS lead to a decrease in soil microbial biomass and an increase in soil respiratory activity as well as in the metabolic quotient (qCO2) of the soils. Denitrification capacity was lowest without the addition of ABS and highest at a medium ABS concentration of 50 μg g–1. Denitrification capacity seems to be highly sensitive to ABS addition at moderate concentrations. From the laboratory results, high rates of denitrification and N2O evolution from fields irrigated with wastewater containing ABS are expected.
    Type of Medium: Electronic Resource
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