ISSN:
1432-069X
Keywords:
Key words IRF-1
;
ICAM-I
;
Tryptophanyl tRNA
;
synthetase
;
IFN-γ
;
Keratinocyte
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Interferon gamma (IFN-γ) is a potent inducer of cell growth arrest in human epidermal keratinocytes. Interferon regulatory factor-1 (IRF-1), an interferon-inducible gene, is a mediator of interferon action. It has also been suggested that IRF-1 may have a functional role as a tumor suppressor gene and may be associated with the antiproliferative effect of IFN-γ. We examined the expression of IRF-1 mRNA in normal human epidermal keratinocytes (NHEK). Northern blot analysis demonstrated an increased expression of IRF-1 mRNA by IFN-γ in NHEKs. This increased expression of IRF-1 mRNA by IFN-γ was not blocked by treatment with cycloheximide, but was abolished by treatment with actinomycin D. In addition, neither pretreatment with TPA, a protein kinase C (PKC) activator, nor treatment with H7, a PKC inhibitor, affected the induction of IRF-1 mRNA by IFN-γ. These results indicate that IFN-γ-induced IRF-1 mRNA in NHEKs is transcriptional and PKC-independent.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s004030050214
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