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  • 1
    Electronic Resource
    Electronic Resource
    Ca2+-Dependent 68-Kilodalton Protease in Familial Alzheimer's Disease Cells Cleaves the N-Terminus of .beta.-Amyloid (1994)
    s.l. : American Chemical Society
    Biochemistry 33 (1994), S. 3941-3948 
    Details
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/bi00179a021
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  • 2
    Electronic Resource
    Electronic Resource
    Higher inductions of twin and single sister chromatid exchanges by cross-linking agents in Fanconi's anemia cells (1982)
    Springer
    Human genetics 〈Berlin〉 60 (1982), S. 233-238 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Twin and single sister chromatid exchanges (SCEs) induced by short treatments with mitomycin C (MC) and 4,5′,8-trimethylpsoralen (TMP)-plus-near ultraviolet light (NUV) were analyzed in colcemid-induced endoreduplicated normal human and typical Fanconi's anemia (FA) fibroblasts with diplochromosomes. The induction rate of twin SCEs that had occurred in the first cycle (S1) after the treatment was 1.7–2.4 times higher in FA cells than in normal cells. The induction rate of single SCEs that had arisen during the second cycle (S2) long after the treatment was also much higher, though less than the twin SCE rate, in FA cells than the almost neglible rate after repair of cross-links and monoadducts in normal cells. These results in FA cells, which specifically lack the first half-excision step of the two-step cross-link repair but retain the normal monoadduct repair, indicate that MC or TMP cross-links remaining unrepaired are indeed responsible for higher inductions of twin (S1 exchange) and single SCEs (S2 exchange). Thus, these findings indicate that Shafer's model of replication bypass for cross-link-induced SCE, which predicts greatly reduced twin SCE formation in FA cells due to half cancellation, is apparently inadequate as such. We present three plausible models, incorporating the ordinary replication model, random unilateral cross-link transfer, and chromatid breakage/reunion, that can account for the probabilistic inductions of single and twin SCEs and even for no SCE formation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00303010
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  • 3
    Electronic Resource
    Electronic Resource
    The two locus control of Leber hereditary optic neurophathy and a high penetrance in Japanese pedigrees (1993)
    Springer
    Human genetics 〈Berlin〉 91 (1993), S. 339-341 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The maternal transmission of Leber hereditary optic neuropathy (LHON) can be explained by the mitochondrial DNA mutation. However, the characteristic mode of inheritance, i.e. male predominance and reduced penetrance with late onset in females, suggests the simultaneous involvement of an X-linked gene in development of optic atrophy. We have assessed such a two-locus model of mitocnondrial and X-linked genes in Japanese LHON pedigrees. The goodness-of-fit test on individual male sibship data with a presumed heterozygous mother from maternal lines showed an excellent fit for the 1:1 segregation of a putative X-linked gene, thus supporting the two-locus model in the Japanese pedigrees tested. A calculated frequency of the X-linked gene was 0.10. We could not determine whether the present value is different from the reported one (= 0.08). On the other hand, the estimated penetrance for a heterozygous female was 0.196±0.039, which was about twice as high as the reported value (=0.111) with a 5% level of significance. Such a high penetrance may primarily arise from a low threshold of LHON manifestation, suggesting the ethnic difference between the LHON pedigrees in Japan and in other countries.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00217353
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  • 4
    Electronic Resource
    Electronic Resource
    Amelioration of the ultraviolet sensitivity of an Escherichia coli recA mutant in the dark by photoreactivating enzyme (1983)
    Springer
    Molecular genetics and genomics 190 (1983), S. 511-515 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary When a recA strain of Escherichia coli is transformed with a multicopy plasmid, pKY1, carrying the phr gene of E. coli, its extreme ultraviolet sensitivity is decreased. Derivatives of pKY1 were prepared in which the phr gene was inactivated by inserting transposon Tn1000. None of the 20 ph - derivatives decreased the UV sensitivity of the recA strain. In an analogous experiment, we obtained 11 derivatives which failed to decrease the UV sensitivity of the recA strain. None of them complemented phr strain. Furthermore, Tn1000 insertion sites in both types of derivatives were mapped in the same region of the plasmid. From these observations, we propose that the E. coli phr gene product has repair activity in the dark.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00331084
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  • 5
    Electronic Resource
    Electronic Resource
    Evidence that the phr + gene enhances the ultraviolet resistance of Escherichia coli recA strains in the dark (1983)
    Springer
    Molecular genetics and genomics 192 (1983), S. 282-284 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary An Escherichia coli recA phr + purA strain was more resistant to ultraviolet radiation than its isogenic derivative recA phr + purA + in the absence of photoreactivating light, whereas their nearly isogenic derivative recA phr showed most UV-induced lethality. The amounts of photoreactivating enzyme (PRE) per cell in the recA phr + purA was higher than in the recA phr + purA +. The recA phr is defective for photoreactivation. Thus, in the recA strain, UV resistance in the dark increased in proportion to the amounts of PRE per cell, suggesting that PRE participates in the process of dark repair of UV-damaged DNA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00327679
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  • 6
    Electronic Resource
    Electronic Resource
    Role of RNA synthesis in DNA replication of synchronized populations of cultured mammalian cellsAbbreviations used: DNA, deoxyribonucleic acid; RNA, ribonucleic acid; TDR, thymidine; UR, uridine; PBS, phosphate-buffered saline; PCA, perchloric acid; G1, the pre-DNA synthesis period; S, the DNA synthesis period; G2, the post-DNA synthesis period; M, mitosis. (1967)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 70 (1967), S. 291-299 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Using the harvesting method of synchronizing L cells, the relationship of RNA synthesis of DNA replication was studied by the use of selective inhibitors of RNA synthesis such as actinomycin D and chromomycin succinate. The synthesis of the early replicating DNA fraction is a process sensitive to the inhibition of RNA synthesis during the G1 period.The synthesis of early replicating DNA was inhibited by chromomycin succinate without affecting the initation of DNA synthesis. However, actinomycin D inhibited the synthesis of early replicating DNA and prevented the initiation of DNA synthesis in 50% of the synchronized cells. However, it was found that the continued synthesis of RNA during the S period is not essential for the synthesis of late replicating DNA.In addition to this specific response of DNA synthesis to the inhibitors of RNA synthesis, another function of early and late replicating DNA was determined relative to the cell viability. Cells synthesizing early replicating DNA were killed more efficiently by chromomycin than at other stages of the cell cycle. This indicates that the early replicating DNA unit plays a more important role in cell reproduction than the late replicating DNA unit.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040700309
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  • 7
    Electronic Resource
    Electronic Resource
    A retarded rate of DNA replication and normal level of DNA repair in Werner's syndrome fibroblasts in culture (1977)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 92 (1977), S. 365-374 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: We investigated the cloning efficiency, DNA repair, and the rate of DNA replication in the skin fibroblasts from patients with Werner's syndrome (WS) of an autosomal recessive premature aging disease. Five WS strains exhibited normal levels of sensitivity toward X-ray and UV killings and repair of X-ray induced single strand breaks of DNA (rejoining) and UV damage to DNA (unscheduled DNA synthesis). The sedimentation of newly synthesizing DNA in alkaline sucrose gradients demonstrated a characteristic feature that only the elongation rate of DNA chains, estimated by the molecular weight increase, was significantly slower during early passages in WS cells than in normal Hayflick Phase II fibroblasts. In addition, plating efficiencies as well as the replicative potentials of five WS strains were more limited than those of normal cells under the identical culture conditions. It seems therefore that at least in the WS cells tested, the slow rate of DNA replication may be more related to the shortened lifespan and enhanced cell death, as manifestation of premature senescence at the cellular level, than be the DNA repair ability.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040920305
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