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  • 1
    Electronic Resource
    Electronic Resource
    Effects of a bleaching agent containing 35% carbamide peroxide on the immunolocalization of cyclin D and p16 (2002)
    Oxford, UK : Blackwell Science Ltd
    Journal of oral rehabilitation 29 (2002), S. 0 
    Details
    ISSN: 1365-2842
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Summary The evidences that p16 and cyclin D alterations occur in the earlier stages of many human cancers and that its immunolocalization can be used to study the cell cycle regulation, coupled with the possibility that chronic use of carbamide peroxide would induce DNA damage and cell cycle alteration, prompted us to analyse the effect of carbamide peroxide on the immunolocalization of these proteins in rat oral mucosa. Ten male Wistar rats were selected and submitted to topical application of 35% carbamide peroxide over buccal mucosa. The procedures were carried out twice a week for 3 months consecutively. The animals were killed after the last treatment and the buccal mucosa was removed and stored at −70 °C. Only distilled water was applied over the buccal mucosa of the control animals. The biotin–streptavidin amplified system was used for identification of cyclin D and p16 antigens and the percentage of basal and suprabasal cells positive for each one were obtained. The results did not show any difference between the experimental and control groups regarding the immunolocalization of cyclin D and p16. In conclusion, the present study showed that chronic use of carbamide peroxide does not induce cell cycle alteration in the oral mucosa of rats.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2842.2002.00944.x
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Chemoluminescence generation and MTT dye reduction by polymorphonuclear leukocytes from periodontal disease patients (1994)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of periodontal research 29 (1994), S. 0 
    Details
    ISSN: 1600-0765
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Alterations of polymorphonuclear leukocytes (PMNs) functions have been reported in patients with severe forms of some periodontal disease. In this study we evaluated the chemoluminescence generation and MTT dye reduction by human PMN in patients with juvenile periodontitis (JP), rapidly progressive periodontitis (RPP) and adult periodontitis (AP) during protein kinase C (PKC) activation or during the phagocytosis of opsonized zymosan. The results demonstrated that only PMNs of JP patients showed a decreased chemoluminescence generation and MTT dye reduction during the phagocytosis of opsonized zymosan (p 〈 0.05). The time to reach the maximal peak during the PKC activation on the chemoluminescence reaction was evaluated and JP PMNs patients demonstrated a depressed value (7.0 ± 0.4 min) compared with healthy volunteers (13.8 ± 0.5 min). The etiology and importance of such cellular alterations in the immunopathogenesis of the periodontal disease are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0765.1994.tb01098.x
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Vascular endothelial growth factor in minor salivary glands: effect of ageing (2002)
    Oxford UK : Blackwell Science Ltd
    Journal of oral rehabilitation 29 (2002), S. 0 
    Details
    ISSN: 1365-2842
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Morphological and physiological age changes are described in human salivary glands. Vascular endothelial growth factor (VEGF) is neoangiogenic growth factor found in normal salivary glands. Considering the neoangiogenic properties of VEGF and its important function in inflammation, repair and, probably, in oral mucosa homeostasis, the purpose of the present study was to evaluate the effect of ageing on the immunolocalization of VEGF in minor salivary glands. Paraffin-embedded tissue blocks containing normal labial salivary glands were retrieved and classified according to the patients’ age in two groups (〈 20 and 〉 40-year-old). The biotin–streptavidin–peroxidase system was used to detect the VEGF antigen. The results demonstrated that the mean level of VEGF immunoreaction in the young group was not statistically different from the old group when compared by the Mann–Whitney U-test (P = 0·54). This may indicate that although salivary flow reduction may develop in old patients, some properties of the salivary glands may not be affected.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2842.2002.00800.x
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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