Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Somatic Hybridization of Nitrate Reductase Deficient Mutants of Nicotiana tabacum by Protoplast Fusion (1978)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 44 (1978), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Protoplasts were isolated from two mutant cell lines of Nicotiana tabacum L. cv. Gatersleben and fused with the aid of polyethylene glycol. Both mutants lacked nitrate reductase and were thus auxotrophic for reduced nitrogen. The fusion resulted in a high frequency of hybrid cells which were detected by their regained ability to grow in media containing nitrate as sole nitrogen source. Thus, the two mutants were found to complement each other in the hybrids. In control experiments, back mutation and cross-feeding were excluded as possible explanations for the occurrence of cell lines utilizing nitrate. A total of 1061 hybrid lines capable of sustained proliferation were isolated. Some of them were further characterized with respect to nitrate reductase activity, chlorate sensitivity, chromosome number, and shoot formation.The results demonstrate that protoplast fusion can be used for the genetic analysis of cell variants of higher plants and that nitrate reductase-deficient mutants provide efficient selective systems for hybrid cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1978.tb08631.x
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Isolation and characterization of cell lines of Nicotiana tabacum lacking nitrate reductase (1978)
    Springer
    Molecular genetics and genomics 161 (1978), S. 67-76 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Chlorate-resistant cell lines were established from survivors after plating allodihaploid cells of Nicotiana tabacum into solid medium containing 20 mM chlorate and amino acids as sole nitrogen source. Data characterizing 9 of the most resistant lines are presented. The mutational origin of these lines was inferred on the basis of the enhancement of the variant frequency by mutagen treatment, and of the persistance of the variant phenotype in cell progeny during growth in the absence of selection for more than 3 years and in plants regenerated from two of the lines. Seven lines completely lacked in vivo nitrate reductase (NR) activity and two lines exhibited low (less than 5% of the wild type) NR activity. The abolition of NR activity was found to be not due to an impaired induction by nitrate. Data reported elsewhere show that one of the NR-negative mutants simultaneously lacks xanthine dehydrogenase activity. This pleiotropic mutation is interpreted to affect the synthesis of a molybdenum-containing cofactor, whereas the 8 other lines carry mutations specifically affecting the synthesis of the NR. Both types of NR-negative mutants were unable to grow on minimal medium containing nitrate as sole nitrogen source, but grew well on amino acids. They proved extremely sensitive to the standard medium containing nitrate and ammonium. Differences between the NR-negative mutants with respect to chlorate resistance suggest that chlorate inhibits cultured N tabacum cells not only via its NR-catalysed conversion to chlorite, but also by NR-independent mechanisms.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00266616
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Genetische Experimente mitin vitro-kultivierten Pflanzenzellen. Methodische Voraussetzungen und Anwendungsmöglichkeiten (1978)
    Springer
    Genetic resources and crop evolution 26 (1978), S. 13-36 
    Details
    ISSN: 1573-5109
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Summary Somatic hybridization and mutant isolation via cell culture are reviewed. The qualification of various plant species for the application of these methods to basic research and plant breeding is discussed. A survey is made of the status of the culture techniques possible in important crop plants.
    Abstract: Краткое содержание предпосылки и возмож ности применения Дается сводка по сома тической гибридизац ии и получению мутантов м етодами клеточных культур in vitro. О бсуждается пригодно сть различных видов растений для пр именения этих методо в в теоретических иссле дованиях и в селекции растений. Дается также обзор со временного состояния применени я этой методики для важ нейших сельскохозяй ственных культурных растений.
    Notes: Zusammenfassung Somatische Hybridisierung und Mutantengewinnung durchin vitro-Kulturtechniken werden referiert. Die Eignung verschiedener Pflanzenarten für eine Anwendung dieser Verfahren in Grundlagenforschung und Pflanzenzüchtung wird diskutiert und ein Überblick über den Stand derin vitro-Kulturtechniken bei bedeutenden landwirtschaftlichen Kulturpflanzen gegeben.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02017330
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...