ISSN:
1432-0738
Keywords:
Lead
;
Ca-EDTA
;
Bone
;
Atomic absorption spectroscopy
;
Laser microbeam mass analysis
;
Electron microbeam X-ray analysis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Two iliac crest needle biopsies were taken from a 43-year-old lead-poisoned woman during and after completion of a Ca-EDTA treatment. By atomic absorption spectroscopy the first and second biopsy were found to contain 56, respectively 41.6 μg lead/g wet tissue. In both biopsies 36% of the lead was extractable in 0.1 N HCl. Electron microbeam X-ray analysis proved to have too low sensitivity for quantitation of the lead in these biopsies. Laser microbeam mass analysis (LAMMA), performed only on the second biopsy, revealed a high and fairly constant residual lead concentration in all bone marrow cell nuclei (approximately 55 μg/g) and a low lead concentration in the cytoplasm of the same cells (4–12 (μg/g). The extracellular bone matrix lead was greatly concentrated in the superficial 3–6 μm osteoid zone of the bony trabeculae and totally absent from deeper parts of the mineralized matrix. The LAMMA results are in good agreement with those of subcellular fractionation experiments and atomic absorption spectroscopy, provided that the relative volume fraction of nucleus and cytoplasm is accounted for. The high residual osteoid lead after completed chelation therapy indicates that lead has a stronger affinity for the organic than the mineral components of bone matrix.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00332490
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