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  • 1
    ISSN: 1615-6110
    Keywords: Leguminosae ; Phaseolus ; Isozymes ; genetic distance ; Lima bean ; rDNA ; RFLP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Genetic variation inPhaseolus lunatus (Lima bean) was investigated at isozyme and DNA levels. Sixty cultivated accessions, including representatives of the Mesoamerican and Andean gene pools and intermediate types, were analyzed for variability at 17 isozyme loci. Some accessions were also examined for restriction fragment length polymorphism (RFLP) at the rDNA level. These data were used to construct two dendrograms showing clear separation in two distinct groups corresponding to each of the gene pools and an intermediate one probably representing a transitional group.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Genetic resources and crop evolution 39 (1992), S. 115-119 
    ISSN: 1573-5109
    Keywords: chickpea ; chromatin characterization ; chromosome banding ; genome evolution ; heterochromatin diversity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The karyotype of Cicer arietinum L. was characterized after the use of chromosome differential staining obtained through various techniques, such as C-banding and fluorochrome staining. It was possible to individually identify all the chromosomes of this species. Moreover the use of fluorochromes with different DNA targets allowed the distinction of at least two types of heterochromatin. The importance of chickpea heterochromatin characterization is discussed in the perspective of evolutionary and breeding studies.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Genetic resources and crop evolution 42 (1995), S. 57-60 
    ISSN: 1573-5109
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Karyomorphological similarities among four species of the genusVigna, V. unguiculata, V. luteola, V. oblongifolia andV. vexillata, were evaluated. Two different approaches were followed: cytomorphometrical determinations, carried out with an automated image analysis system to improve resolution, yielded some karyotypical indices; the distribution of heterochromatin bands was interpreted using a recently reported algorithm. Both methods led to similar results: the most karyologically similar species toV. unguiculata appears to beV. luteola, whileV. oblongifolia appears to be the least related one.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Genetic resources and crop evolution 42 (1995), S. 347-352 
    ISSN: 1573-5109
    Keywords: sheterochromatin ; rate of allogamy ; microevolution ; seed proteins ; Vicia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary 27 accessions ofVicia benghalensis from different geographical origins constitute the pool on which the present study was performed. Genetic variation among the samples was biochemically and cytologically evaluated: seed storage protein profiles and C-banded karyotypes were analysed from single individuals of each accession. SDS-PAGE has shown the possibility to divide the samples into two groups, each characterized by specific protein profiles. The two patterns were indicated as A and B. From individual seed electrophoresis it was ascertained that samples possessing the pattern A showed a low level of individual variation, while those possessing the pattern B were highly polymorphic, thus suggesting differences in the allogamic rate. The cytological analysis demonstrated the presence of two groups of accessions, one being much richer in heterochromatin as evidenced by C-banding (H+) than the other (H-). The analysis of biochemical and karyological data showed a constant association between pattern A and karyotype H− and between pattern B and karyotype H+. On the basis of these results it is proposed to considerV. benghalensis as a highly heteromorphic species, in which two groups may be identified.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1573-5060
    Keywords: wild wheats ; Aegilops ventricosa ; chromosomal polymorphism ; cytogenetics ; seed proteins ; evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Five samples of Aegilops ventricosa (2n=4x=28, genome formula DDNN) from different geographical origins, were crossed in a diallelic scheme. Metaphase I chromosome pairing of the hybrids, accounting for all the possible genetic combinations, was analysed. Only bivalents were formed in some hybrids, while multivalents were scored in other ones. Seed storage proteins, gliadins and albumins, were also analysed by means of polyacrylamide gel electrophoresis. Based on the presence of multivalents in hybrids, and on the differences in seed protein profiles, the samples could be grouped into two clusters. Meiosis was regular in hybrids obtained within samples of the same group, while multivalents were present in hybrids involving a sample of one group and one of the other. The evolutionary trends in Ae. ventricosa are discussed.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1573-6849
    Keywords: chromosome constitution ; Dasypyrum villosum ; heterochromatin structure ; RE-banding ; Triticeae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The distribution of C-banded heterochromatin was determined in an inbred line ofDasypyrum villosum. Practically no difference in chromosome morphology or band distribution could be observed within the chromosomes of the same pair. Heterochromatin bands, revealed by Giemsa banding, were characterized by means of their differential reaction to fluorochromes, silver staining andin situ digestion with different restriction endonucleases. The results clearly indicate that inD. villosum two different classes of heterochromatin with different chromosomal local-ization exist: one is evidenced by both C-banding and DAPI staining and has mainly telomeric distribution, the other is evidenced only by C-banding and has mainly centromeric distribution.
    Type of Medium: Electronic Resource
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