ISSN:
0377-0486
Keywords:
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
,
Physics
Notes:
A simple and fully computer-controlled backscattering incident circular polarization Raman optical activity (ICP ROA) instrument suitable for chioptic studies of biologically significant molecules in aqueous solution is described. It consists of a fast single-stage monochromator equipped with a holographic edge filter, a highly efficient holographic difiaction grating and a thinned back illuminated thermoelectrically cooled charge-coupled device detector as the key spectral elements. A longitudinal electro-optic modulator is employed to switch between orthogonal circular polarization states in the incident laser radiation within a backscattering geometry. A thick Lyot depolarizer is used for the depolarization of the backscattered Raman radiation. High-quality backscattering ICP ROA spectra of (1S)-( - )-trans-pinane, (1R)-( + )-trans-pinane, L-alanine, L-alanyl-L-alanine, lysozyme, D-glucose, α-D-cyclodextrin and 2′-deoxycytidine are presented as typical examples of the excellent performance characteristics.
Additional Material:
9 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jrs.1250230706
Permalink
