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  • 1
    Electronic Resource
    Electronic Resource
    Genetic mapping of the barley Rrs14 scald resistance gene with RFLP, isozyme and seed storage protein markers (2000)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 119 (2000), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The scald susceptible barley cultivar ‘Clipper’ and a third-backcross (BC3) line homozygous for the Rrs14 scald resistance gene that originally came from Hordeum vulgare ssp. spontaneum were grown in replicated field trials. The level of resistance that Rrs14 confers against field populations of the pathogen Rhynchosporium secalis, the causal agent of scald disease, was evaluated. The Rrs14 BC3 line exhibited 80% and 88% less leaf damage than ‘Clipper’ in 1995 and 1996, respectively. Given this effectiveness of Rrs14, research was undertaken to identify a linked marker locus suitable for indirect selection of Rrs14. Based on linkage to a set of previously mapped loci, Rrs14 was positioned to barley chromosome 1H between the seed storage protein (hordein) loci Hor1 and Hor2, approximately 1.8 cM from the latter locus. The Hor2 locus is thus an ideal codominant molecular marker for Rrs14. The tight linkage between Rrs14 and Hor2 and the availability of alternative biochemical and molecular techniques for scoring Hor2 genotypes, permits simple indirect selection of Rrs14 in barley scald resistance breeding programmes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1439-0523.2000.00456.x
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Two tomato non-symbiotic haemoglobin genes are differentially expressed in response to diverse changes in mineral nutrient status (2003)
    Oxford, UK : Blackwell Science Ltd
    Plant, cell & environment 26 (2003), S. 0 
    Details
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The role of plant non-symbiotic haemoglobins remains undefined, but recent findings suggest a possible role in plant nitrate nutrition. This study sought to characterize patterns of gene expression for two tomato (Lycopersicon esculentum L.) non-symbiotic haemoglobin genes, and to examine how diverse changes in nutrient status influences the expression of these two genes. The deduced amino acid sequences of the genes examined, SOLly GLB1 and SOLly GLB2, are 55% identical. SOLly GLB1 mRNA is present in highest abundance in roots and older stems, whereas SOLly GLB2 mRNA abundance is highest in leaves, but detectable in other tissues including roots. The SOLly GLB1 mRNA levels increased rapidly in roots in response to each nutrient treatment examined in hydroponic culture, including the individual removal of phosphate, potassium and iron from the culture medium, as well as by the addition of nitrate. In contrast, the levels of SOLly GLB2 mRNA were not significantly altered in response to these same treatments. These results are the first report that non-symbiotic haemoglobin gene expression can be influenced by a broad range of changes both in mineral macronutrient and micronutrient status, suggesting a previously unrecognized generic role for non-symbiotic haemoglobins in processes associated with mineral nutrient nutrition.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-3040.2003.01002.x
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    The effect of combining scald resistance genes on disease levels, yield and quality traits in barley (1996)
    Springer
    Theoretical and applied genetics 93 (1996), S. 361-366 
    Details
    ISSN: 1432-2242
    Keywords: Key words Pyramiding  ;  Hordeum vulgare ssp. spontaneum  ;  Rhynchosporium secalis  ;  Backcross lines  ;   Isozyme marker genes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Pairwise combinations of genes for resistance to scald in barley were developed using linked isozyme markers to test whether such combinations conferred improved resistance to the pathogen, Rhynchosporium secalis. The resistance genes originally derived from Hordeum vulgare ssp. spontaneum. The combinations were bred into an essentially similar genetic background because the scald-susceptible, Australian barley cultivar ‘Clipper’ was the recurrent backcross parent in their ancestry. In field tests of the recombinants over 2 years, disease levels were lower in three of six doubly resistant lines than in backcross lines carrying a single resistance gene, which in turn were less diseased than either ‘Clipper’ or recombinants that lacked the marked resistance genes. All resistant lines significantly outyielded ‘Clipper’ but did not themselves differ significantly. Lines resistant to scald had significantly higher grain size and grain weight. Gains for malt yield of about 1% were detected in the higher disease environment. Resistance was not accompanied by any obvious “cost” in terms of yield or quality. Protection against scald is therefore a significant requirement for new malting barley cultivars in scald-prone areas.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050289
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    The effect of combining scald resistance genes on disease levels, yield and quality traits in barley (1996)
    Springer
    Theoretical and applied genetics 93 (1996), S. 361-366 
    Details
    ISSN: 1432-2242
    Keywords: Pyramiding ; Hordeum vulgare ssp ; spontaneum ; Rhynchosporium secalis ; Backcross lines ; Isozyme marker genes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Pairwise combinations of genes for resistance to scald in barley were developed using linked isozyme markers to test whether such combinations conferred improved resistance to the pathogen, Rhynchosporium secalis. The resistance genes originally derived from Hordeum vulgare ssp. spontaneum. The combinations were bred into an essentially similar genetic background because the scald-susceptible, Australian barley cultivar ‘Clipper’ was the recurrent backcross parent in their ancestry. In field tests of the recombinants over 2 years, disease levels were lower in three of six doubly resistant lines than in backcross lines carrying a single resistance gene, which in turn were less diseased than either ‘Clipper’ or recombinants that lacked the marked resistance genes. All resistant lines significantly outyielded ‘Clipper’ but did not themselves differ significantly. Lines resistant to scald had significantly higher grain size and grain weight. Gains for malt yield of about 1 % were detected in the higher disease environment. Resistance was not accompanied by any obvious “cost” in terms of yield or quality. Protection against scald is therefore a significant requirement for new malting barley cultivars in scald-prone areas.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223177
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Inheritance and chromosome locations of scald-resistance genes derived from Iranian and Turkish wild barleys (1997)
    Springer
    Theoretical and applied genetics 94 (1997), S. 1086-1091 
    Details
    ISSN: 1432-2242
    Keywords: Key words Hordeum vulgare ; Genetic mapping ; Rhynchosporium secalis ; Multiple resistance genes ; Isozymes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  A set of advanced backcross barley lines derived from crosses between cv Clipper and different Iranian and Turkish wild barleys, which are homozygous for particular isozyme-marked donor intervals, was screened for resistance to barley scald. Eight lines that consistently exhibited scald resistance were identified, and genetic analysis indicated that single dominant genes encoded resistance in five of the lines, single recessive genes were present in two lines, and a pair of unlinked, dominant genes encoded the resistance in the last line. Linkage between the scald-resistance gene and the isozyme marking the introgressed donor chromosome interval was detected in four lines, allowing the chromosome locations of these resistance genes to be determined. One such resistance gene resides on barley chromosome 5, to which no other scald-resistance genes have been mapped; this gene has been designated Rrs14. A survey of the effectiveness of the eight resistance genes against a set of virulent pathotypes of the scald pathogen revealed that four of the lines were completely resistant to all of them. In two instances, the recovery of more than one scald-resistance gene from a single original donor parent could be demonstrated. These scald-resistance genes should provide additional opportunities for breeding programs that aim to develop scald-resistant barley cultivars.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050519
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Towards an understanding of the molecular basis of plants K+ transport: Characterization of cloned K+ transport cDNAs (1993)
    Springer
    Plant and soil 155-156 (1993), S. 115-118 
    Details
    ISSN: 1573-5036
    Keywords: cDNA ; cloning ; heterologous expression ; high-affinity K+ transport ; K+ channel ; K+ transport ; oocyte ; root ; yeast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Recently, two K+-transport cDNAs, KAT1 and AKT1, were cloned in Arabidopsis thaliana. These cDNAs had structural similarities to K+ channel genes in animals, and also conferred the ability for growth on micromolar levels of K+ when expressed in K+ transport-defective yeast mutants. In this study, we examined the possibility that KAT1 encodes the high-affinity K+ transport system that has been previously characterized in plant roots, by studying the concentration-dependent kinetics of K+ transport for KAT1 expressed in Xenopus oocytes and Saccharomyces cerevisiae. In both organisms, the K+ transport system encoded by KAT1 yielded Michaelis-Menten kinetics with a high Km for K+ (35 mM in oocytes, 0.6 mM in yeast cells). Furthermore, Northern analysis indicated that KAT1 is expressed primarily in the Arabidopsis shoot. These results strongly suggest that the system encoded by KAT1 is not a root high-affinity K+ transporter.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00024997
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    Paper (German National Licenses)
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