ISSN:
1432-2242
Schlagwort(e):
Key words Self-incompatibility
;
S-haplotype
;
S-locus glycoprotein
;
Immunochemical analysis
;
Brassica oleracea var ‘botrytis’
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
Notizen:
Abstract Self-incompatibility in the Brassicaceae is controlled by the single multiallelic S-locus which contains at least two genes expressed in the stigma, the SLG (S-locus glycoprotein) and SRK (S-locus receptor kinase) genes. The presence of two transcriptional units at the S-locus led to the use of the term S-haplotype to define allelic forms of the S-locus. On the basis of sequence data obtained for SLG and SRK from different S-haplotypes, two classes of S-haplotypes have been described in Brassica. Here, we report the identification of S-haplotypes in 126 plants representing 82 open-pollinated cultivars of B. oleracea var ‘botrytis’ (cauliflower) by means of an immunochemical analysis of SLG products. The antibodies used enabled class I and class II S-haplotypes to be discriminated. Ten different S-haplotypes were identified, eight of class I, only one belonging to class II and another one for which no SLG products were detected by our antibodies. In heterozygous plants containing the class II S-haplotype associated with some particular class I S-haplotypes, specific modifications of class II SLGs were found. Pollen tube counts and seed set were used and compared to assess the self-incompatibility phenotypes. SC, a class I haplotype, was always found to be associated with a fully self-compatible phenotype. Half of the plants analyzed possessed the class II S-haplotype. Plants homozygous for this haplotype showed various levels of self-incompatibility, from highly self-incompatible to fully self-compatible. These results are discussed with specific reference to, F1 hybrid breeding.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/s001220050421
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