ISSN:
0269-3879
Keywords:
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Medicine
Notes:
A method for the preparation of a new packing material for high performance liquid chromatography by bonding an anti-interferon monoclonal antibody to the surface of silica gel is described. The high coupling efficiency and activity of the interferon-αA-monoclonal antibody (IFN-αA-McAb) column were obtained by activated diol-silica gel with an activating agent. After purification with this packing material, the specific activity of recombinant human interferon-αA rose by up to 1.03 × 107IU/mg protein and the purification efficiency was increased by approximately 100 times.
Additional Material:
3 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bmc.1130070608
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