Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    An internal standard improves the reliability of transient expression studies in plant protoplasts (1991)
    Springer
    Plant cell reports 10 (1991), S. 401-405 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Transient expression of foreign genes introduced on a plasmid into isolated plant protoplasts is widely used to study the control of gene expression. Unfortunately, many experimental variables implicated in this technique are difficult or impossible to control, resulting in a disturbing degree of variability between otherwise identical experiments. We have studied the co-expression of two constitutively expressed genes located on the same plasmid. This has allowed us to identify the lot of plasmid DNA as an important source of variation, along with the protoplast lot. Plasmid DNA concentration was found to be of minor importance. Since the variation of expression level of the two genes was identical for the two genes in all experiments, we propose the use of an internal standard in all comparative transient expression studies, which allows the reduction of the variation between experiments by one order of magnitude.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232611
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    cDNA nucleotide sequence and expression of a maize cytoplasmic ribosomal protein S13 gene (1993)
    Springer
    Plant molecular biology 21 (1993), S. 701-704 
    Details
    ISSN: 1573-5028
    Keywords: cDNA sequence ; ribosomal protein S13 ; gene expression maize
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The complete amino acid sequence of a cytoplasmic ribosomal protein S13 of maize was deduced from the cDNA isolated from a maize cDNA library. The encoded protein is 151 amino acids long and shows a homology of 73% with the corresponding protein S13 of rat. Southern blots analysis shows that the maize protein S13 is encoded by a small multigene family conserved in plant species closely related to maize. The S13 RNAs accumulate preferentially in proliferating tissues and cells and their transcription occurs in parallel to the DNA synthesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00014553
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Nucleotide sequence and expression of two cDNA coding for two histone H2B variants of maize (1992)
    Springer
    Plant molecular biology 20 (1992), S. 581-588 
    Details
    ISSN: 1573-5028
    Keywords: histone variants ; cDNA ; expression ; maize
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The complete amino acid sequences of two variants of histone H2B of maize were deduced from the cDNAs isolated from a maize cDNA library. The two encoded proteins are 150 (H2B(1)) and 149 (H2B(2)) amino acids long and shows the classical organization of H2B histones. The hydrophobic C-terminal region is highly conserved as compared to that of the animal counterparts with only 21 changes (13 conservative) among the 90 residues. Between the N-terminal part and the C-terminal region we note the presence of a basic cluster (9 residues) characteristic of histones H2B. The N-terminal third is extended as compared to the animal consensus H2B and has the same size as the H2B histone of wheat. Up to 9 acidic residues and a five time repeated pentapeptide PA/KXE/KK are present in this region. Southern-blot hybrization showed that the H2B histones are encoded by a multigenic family like the other core histones (H3 and H4) of plants. The general expression pattern of these genes was not significantly different from that of the H3 and H4 genes neither in germinating seeds nor in different tissues of adult maize.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00046443
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Nuclease sensitivity and functional analysis of a maize histone H3 gene promoter (1993)
    Springer
    Plant molecular biology 22 (1993), S. 1007-1015 
    Details
    ISSN: 1573-5028
    Keywords: chromatin ; histone promoter ; hypersensitive sites ; nuclease accessibility ; nucleosomes ; transgenic plants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A 1 kb region of a maize H3 histone gene promoter has been analysed at a structural and functional level. Micrococcal nuclease digestion of isolated nuclei showed that the promoter region is organized into nucleosomes but a zone extending over approximately one nucleosome (20 to 230 bp upstream of the TATA box) displays remarkable accessibility to digestion. Three DNase I-hypersensitive sites were found within this zone at the vicinity of consensus sequences, some of which are already known to act ascis elements. This promoter region is able to direct faithful expression of the GUS reporter gene in meristematic tissues of transgenic tobacco plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00028973
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Organ-specific expression of different histone H3 and H4 gene subfamilies in developing and adult maize (1991)
    Springer
    Plant molecular biology 17 (1991), S. 935-940 
    Details
    ISSN: 1573-5028
    Keywords: expression ; germination ; histone genes ; maize ; subfamilies ; tissues
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The steady-state levels of H3 and H4 mRNAs transcribed from three H3 and two H4 multigene subfamilies were studied during germination and in different organs of maize. During germination the five subfamilies are expressed in parallel to DNA synthesis, but a 5-fold difference in the quantity of mRNAs transcribed per gene copy was found from our subfamily to another. In adult plants H3 and H4 mRNA levels are highest in organs containing meristematic tissues but also high in non-proliferating tissues. No strict tissue specificity expression could be detected but some subfamilies show preferential expression in some tissues.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00037077
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    Protein complexes binding to cis elements of the plant histone gene promoters: multiplicity, phosphorylation and cell cycle alteration (1997)
    Springer
    Plant molecular biology 33 (1997), S. 367-379 
    Details
    ISSN: 1573-5028
    Keywords: cell cycle ; histone transcription ; Nicotiana tabacum BY2 ; trans-acting factors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The S phase-specific expression of histone genes provides an interesting model for studying activation of gene transcription during the cell cycle. In plants, however, trans-acting factors responsible for histone gene transcription are poorly documented. Using combined gel shift, UV cross-linking and competition analysis, we carried out a systematic study to identify and characterize proteins binding with the previously established cis elements of the plant histone gene promoters. Nuclear extracts prepared from the highly synchronizable tobacco BY2 cells were used. We confirmed the presence of proteins binding to the hexamer (ACGTCA) motif which has been previously identified as the binding site of wheat HBP-1 proteins. Interestingly, multiple proteins were found to bind specifically with the nonamer (CAATCCAAC) element and their DNA-binding activity was abolished upon in vitro protein phosphatase treatment. This later result imply phosphorylation/dephosphorylation as a potential post-translational control for DNA-binding activity of nonamer-binding proteins. In addition, the DNA-binding activity of these nonamer-binding proteins was found to be positively correlated with the S phase-specific expression of the histone genes in the synchronized cells, suggesting their function in the activation of transcription during the G1/S transition. Finally, several proteins were observed to bind specifically with an A/T-rich hexamer (TAATAT) motif. Their DNA-binding activity, however, was insensitive to phosphatase activity in vitro and relatively constitutive during the cell cycle. This A/T-rich hexamer as a new cis-acting element of plant histone genes is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005797104536
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 7
    Electronic Resource
    Electronic Resource
    Fractionation and characterization of histones from barley (Hordeum vulgare) leaves (1983)
    Springer
    Plant molecular biology 2 (1983), S. 207-220 
    Details
    ISSN: 1573-5028
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Histones were extracted from purified nuclei isolated from four cereals,viz. barley (Hordeum vulgare), wheat(Triticum aestivum), Aegilops squarrosa and corn (Zea mais), and from tobacco (Nicotiana tabacum). Analysis of the histones on SDS gels showed complex electrophoretic patterns with one species of both H3 and H4, one to three species of H1 and two to five species of H2. Judged from the electrophoretic patterns, the histones from barley, wheat and Aegilops are identical but different from those of corn with respect to H2. Like tobacco, corn showed two H2 components, whereas barley, wheat and Aegilops showed five H2 components. SDS gel electrophoresis of histones extracted from buds and roots of germinating seeds at different steps of germination and from different parts of ten-day-old leaves revealed that the existence in barley of multiple histone 2 variants is not restricted to any particular stage of differentiation of barley. Histones from barley leaves were resolved into four fractions by Biogel P-100 gel filtration and histones 2 were further fractionated by their differential solubility in HCl-ethanol. Each of these five fractions (H1, H3, H4, H2A and H2B, respectively) were characterized by electrophoresis on SDS or Triton-acid-urea gels and by their amino acid compositions as compared with the homologous histones of calf thymus and chicken erythrocytes. This revealed the following: i) H3 and H4 are strictly analogous to their animal counterparts. However, H4 has an unexplained lower electrophoretic mobility in Triton-containing acid-urea gels. ii) H1 contains three components with lower electrophoretic mobilities than H1 from erythrocytes, contains more alanine than lysine and has a lower ratio of basic to acidic residues. iii) Both H2A and H2B contain at least four variants each, with higher molecular weights than in animals and higher lysine to arginine ratios. H2A variants comigrate in acid-urea-Triton gels with chicken erythrocytes H2A, whereas H2B migrate much slower. It was concluded that the presence of multiple major variants of H2A and H2B is a frequent but not universal feature in cereals. The existence of these variants is not restricted to the embryonic stage as previously suggested for wheat (31).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01578380
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 8
    Electronic Resource
    Electronic Resource
    Functional analysis of the promoter region of a maize (Zea mays L.) H3 histone gene in transgenic Arabidopsis thaliana (1998)
    Springer
    Plant molecular biology 37 (1998), S. 275-285 
    Details
    ISSN: 1573-5028
    Keywords: cell cycle ; cis-elements ; histone promoter ; maize ; meristems ; transgenic Arabidopsis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A 1023 bp fragment and truncated derivatives of the maize (Zea mays L.) histone H3C4 gene promoter were fused to the ß-glucuronidase (GUS) gene and introduced via Agrobacterium tumefaciens into the genome of Arabidopsis thaliana. GUS activity was found in various meristems of transgenic plants as for other plant histone promoters, but unexplained activity also occurred at branching points of both stems and roots. Deletion of the upstream 558 bp of the promoter reduced its activity to an almost basal expression. Internal deletion of a downstream fragment containing plant histone-specific sequence motifs reduced the promoter activity in all tissues and abolished the expression in meristems. Thus, both the proximal and distal regions of the promoter appear necessary to achieve the final expression pattern in dicotyledonous plant tissues. In mesophyll protoplasts isolated from the transformed Arabidopsis plants, the full-length promoter showed both S phase-dependent and -independent activity, like other plant histone gene promoters. Neither of the 5′-truncated nor the internal-deleted promoters were able to direct S phase-dependent activity, thus revealing necessary cooperation between the proximal and distal parts of the promoter to achieve cell cycle-regulated expression. The involvement of the different regions of the promoter in the different types of expression is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005909703600
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 9
    Electronic Resource
    Electronic Resource
    Subcellular changes during isolation and culture of tobacco mesophyll protoplasts (1975)
    Springer
    Protoplasma 84 (1975), S. 31-41 
    Details
    ISSN: 1615-6102
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Mesophyll protoplasts isolated by treating tobacco leaves with macerozyme and cellulase were cultured in a modifiedMurashige, andSkoog medium (1962) containing 1% agar. Changes occurring in the cells during isolation and culture were followed by light and electron microscopy. During protoplast preparation, pseudocrystals appeared in the chloroplasts and spherical portions of the chloroplast stroma became surrounded by membranes and cut out into the cytoplasm. During culture, the chloroplasts lost three quarters of their volume, thylacoids were reduced and pseudocrystals disappeared. The cytoplasm at first became vacuolated and poor in ribosomes but, by the third day of culture, it was enriched in membranes and polyribosomes, indicating high metabolic activity. In the cells as a whole there were two types of change; first, a shock phase consequent upon protoplast isolation and second, a phase of very rapid dedifferentiation to a meristematic state.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02075941
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 10
    Electronic Resource
    Electronic Resource
    Organization of the histone H3 and H4 multigenic families in maize and in related genomes (1989)
    Springer
    Molecular genetics and genomics 219 (1989), S. 404-412 
    Details
    ISSN: 1617-4623
    Keywords: Maize ; H3-H4 multigenic families ; Genomic organization ; Related genomes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Five cloned histone H3 and H4 genes from maize have specific 5′ non-transcribed regions. Blot hybridization of each 5′ region to DNA from different maize inbred lines showed that the H3 and H4 multigenic families are organized into subfamilies. Each subfamily has a specific environment and contains a different (from 4–16) number of gene copies. H3 and H4 subfamilies with similar environments as those found in maize were shown to exist in the genomes of more or less related plants, including perennial teosinte, sorgho, sugar cane and Coïx. Such observations may contribute to establishing phylogenetic relationships at a molecular level between different plants and thus highlight some of the evolutionary mechanisms of the genomes of higher plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00259613
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...