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  • 1
    Electronic Resource
    Electronic Resource
    Microdroplet culture (1978)
    Springer
    Naturwissenschaften 65 (1978), S. 158-159 
    Details
    ISSN: 1432-1904
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00440353
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    “Arabidobrassica”: A novel plant obtained by protoplast fusion (1980)
    Springer
    Planta 149 (1980), S. 112-117 
    Details
    ISSN: 1432-2048
    Keywords: Arabidopsis ; Brassica ; Chromosomal elimination ; Hybridization, somatic ; Somatic hybridization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Using somatic hybrid cell lines Arabidopsis thaliana+Brassica campestris, obtained by cloning individual protoplast-fusion products as starting material, shoots and flowering plants have been regenerated. Cytological, biochemical, and morphological analyses indicate that genetic material of both species is present in the resultant plants. Shoots and plants obtained from different lines and different regeneration events differed morphologically and genetically. Most regenerants show morphological abnormalities and unusual organizational patterns. Flowering forms have so far been sterile. “Asymmetric” hybrids (i.e., hybrids bearing most genetic material of one of the parent species and only few chromosomes of the other) were more regular in morphology. The results represent the first case of intergeneric-intertribal hybridization of flowering plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00380870
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  • 3
    Electronic Resource
    Electronic Resource
    Analysis of characters coded for by T-DNA using hybridization between tumorous and normal plant cells (1986)
    Springer
    Plant cell reports 5 (1986), S. 352-355 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In somatic hybrids between tumourous Nicotiana tabacum (B6S3) and normal mesophyll Atropa belladonna cells, the following traits, directly or indirectly connected with T-DNA gene expression and tumourous growth, were analysed: lysopine dehydrogenase activity (LpDH), shoot suppression, root suppression, ability to grow on media with D-lactose as a sole carbon source and resistance to 2-aminoethylcysteine, 5-bromodeoxyuridine and 5-methyltryptophan. Dominant (semidominant) expression was observed for all but one trait studied, e.g. shoot suppression which behaved as a recessive character.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00268600
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    “Arabidobrassica”: Plant-genome engineering by protoplast fusion (1979)
    Springer
    Naturwissenschaften 66 (1979), S. 547-554 
    Details
    ISSN: 1432-1904
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Natural Sciences in General
    Notes: Abstract After fusion of somatic cells (isolated protoplasts) of Arabidopsis thaliana and Brassica campestris, plants could be regenerated in which genetic material from parents of taxonomically different tribes is combined. Between these regenerants asymmetric hybrids have also been obtained, in which one parental genome is represented by reduced chromosome numbers. Results concerning the analysis and genesis of these plants are used to discuss the question whether such monster plants are of any importance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00368808
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  • 5
    Electronic Resource
    Electronic Resource
    Hybrid cell lines Arabidopsis thaliana + Brassica campestris: No evidence for specific chromosome elimination (1978)
    Springer
    Molecular genetics and genomics 165 (1978), S. 257-264 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Protoplasts from callus tissue of Arabidopsis thaliana and from leaves of Brassica campestris were fused using the PEG-high pH-high Ca++-technique. Single dividing fusion products were isolated mechanically and cultured further in 1 μl microdroplets. 31 cell lines each originating from a single fusion event have been obtained. Cytological analysis of 6 cell lines was conducted 4–7 months after isolation. All metaphases examined contained chromosomes of both species. Both Arabidopsis and Brassica specific chromosomes were still retained in cells of 7 month old cultures. Reconstituted di- and multiconstrictional chromosomes were also observed. Biochemical analysis of 12 cell lines was performed in 6–7-month old cultures. Electrophoresis on PAG and specific staining for esterase, lactate dehydrogenase, and peroxidase activities revealed isozymes of both parents to be present in all cell lines. The data obtained are interpreted as evidence for retention and functional activity in hybrid cells of specific chromosomes from each species for at least 7 months of culture.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00332524
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    Paper (German National Licenses)
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