ISSN:
0730-2312
Keywords:
mouse interferon alpha
;
interferon production
;
CHO cells
;
antiviral activity
;
growth resistance
;
1-8
;
2-5A synthetase
;
interferon-stimulated genes 15k
;
54k
;
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Chemistry and Pharmacology
,
Medicine
Notes:
CHO cell lines that constitutively produce the murine interferon-α (IFN-α) subspecies α4 and α6 were constructed. The producer cell lines were protected against viral (vesicular stomatitis virus) infection by the IFN species secreted, but were resistant to the growth inhibitory activity of the IFN species. As compared with α4, the α6 protein displayed a high antiproliferative activity when added to normal CHO cells, which correlates completely with the high antiviral activity of a6 on these cells. Three messenger ribonucleic acid (mRNA) species, which are normally induced in CHO cells by IFN treatment (1-8, 2-5A synthetase, and ISG 15) were constitutively present in CHO producer cell lines. The level of another mRNA (ISG 54), however, was very low in the producer cells as compared with its expression in short-term IFN-treated cells. These data indicate that 1-8, 2-5A synthetase and ISG 15 are not involved in the antigrowth activity of IFN in this system, but rather suggest a function of ISG 54 in this respect.
Additional Material:
3 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jcb.240380406
Permalink
