ISSN:
1432-203X
Keywords:
Pisum sativum
;
transformation
;
regeneration
;
phosphinothricin
;
Agrobacterium tumefaciens
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A reliable Agrobacterium tumefaciens-mediated transformation method has been developed for peas (Pisum sativum) using immature cotyledons as the explant source. Transgenic plants were recovered from the four cultivars tested: Bolero, Trounce, Bohatyr and Huka. The method takes approximately 7 months from explant to seed-bearing primary regenerant. The binary vector used carried genes for kanamycin and phosphinothricin resistance. Transformed pea plants were selected on 10 mg/l phosphinothricin. The nptII and bar genes were shown to be stably inherited through the first sexual generation of transformed plants. Expression of the phosphinothricin-resistance gene in the transformed plants was demonstrated using the ‘Buster’ (=‘Basta’) leaf-paint test and the phosphinothricin acetyl transferase enzyme assay.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00193730
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