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  • 1
    Electronic Resource
    Electronic Resource
    Status incongruence and serum cholesterol in an english general practice
    Amsterdam : Elsevier
    Social Science & Medicine 34 (1992), S. 757-762 
    Details
    ISSN: 0277-9536
    Keywords: general practice ; serum cholesterol ; status incongruence
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0277-9536(92)90362-T
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Rapid growth in plant cell culture
    Amsterdam : Elsevier
    Trends in Biotechnology 6 (1988), S. 233-234 
    Details
    ISSN: 0167-7799
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0167-7799(88)90052-2
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Use of fluorescein diacetate (FDA) to determine ploidy of in vitro watermelon shoots (1999)
    Springer
    Plant cell, tissue and organ culture 58 (1999), S. 199-203 
    Details
    ISSN: 1573-5044
    Keywords: triploid watermelon ; seedless watermelon ; tetraploid watermelon ; plant breeding ; somaclonal variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Ploidy of watermelon [Citrullus lanatus (Thunb.) Matsum. and Nakai shoots and plantlets was estimated by painting the lower epidermis of intact in vitro-derived leaves with fluorescein diacetate (FDA) and observing fluorescence of guard cell chloroplasts with a microscope and UV light. Leaves from in vitro shoot-tip cultures of known diploid cultivars and tetraploid breeding lines were used to establish the mean number of chloroplasts per guard cell pair. Leaves from diploid and tetraploid shoot cultures had 9.7 and 17.8 chloroplasts per guard cell pair, respectively. This method then was used to estimate the ploidy of shoots regenerated from cotyledon explants of the diploid cultivar Minilee. Approximately 11% of the 188 regenerated shoots were classified as tetraploid during in vitro culture. Putative tetraploids were transplanted to the field and self-pollinated. About 45% of tetraploids identified in vitro produced fruit and viable seed. Chloroplast counts of R1 progeny were used to confirm their ploidy. All of the putative diploids were confirmed diploid and all putative tetraploids proved to be non-chimeric true breeding tetraploids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006371516394
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    Paper (German National Licenses)
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