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  • 1
    Electronic Resource
    Electronic Resource
    Genetic changes induced in higher plant cells by a laser microbeam (1990)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 79 (1990), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Introducing foreign genes into higher plants has proved to be complicated, with the exception of transformation of protoplasts of some plants (Negrutiu et al. 1987). In particular, culture of protoplasts and regeneration to plants are difficult in many monocotyledonous crops. Therefore, it would be desirable to avoid extensive tissue culture by introducing cloned genes directly into cells. A laser microbeam can perforate plant cell walls, thus facilitating uptake of genes into cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1990.tb05885.x
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  • 2
    Electronic Resource
    Electronic Resource
    Inhibition of hyaluronidase by dextransulfate and its possible application in anticancer treatment (1983)
    Springer
    Journal of cancer research and clinical oncology 105 (1983), S. 189-190 
    Details
    ISSN: 1432-1335
    Keywords: Dextransulfate ; Hyaluronidase ; Connective Tissue ; Tumor growth ; Metastasis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Hyaluronidase is competitively inhibited by dextransulfate. Hyaluronidase depolymerizes the amorphous component of the interstitial connective tissue and therefore supports tumor spread and metastasis. These processes may be reduced by dextransulfate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00406931
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  • 3
    Electronic Resource
    Electronic Resource
    Early Phase Karyotype Analysis of Chromosome Segregation After Formation of Mouse–Mouse Hybridomas with Chromosome Painting Probes (2000)
    Springer
    Chromosome research 8 (2000), S. 37-44 
    Details
    ISSN: 1573-6849
    Keywords: chromosome number ; chromosome painting ; FISH ; hybridomas ; mouse
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract FISH analysis with chromosome painting probes allows, better than karyotyping after Giemsa banding, the study of chromosome segregation after hybridoma formation. FISH is particularly useful for intraspecies hybrids and allows visualization of small chromosome fragments. Cell hybrids were constructed between P3 × 63Ag8.653 mouse myeloma cells and lymphocytes from BALB/c mice by PEG fusion and by selection in hypoxanthine–azaserine medium. Three hybridomas (A4, D8, F10) were selected and, after cloning, the cells were cultivated in vitro over a period of 28 days. During this time in culture, air-dried metaphase spreads were prepared by standard methods. For FISH chromosome painting, digoxigenin- and biotin-labeled mouse chromosome painting probes and rhodamine–antidigoxigenin antibodies and fluorescein–avidin were used for dual color detection. Total chromosome numbers and the numbers of mouse chromosomes 1, X, 6 and 12 were estimated as function of days in culture. Mean chromosome numbers of 78 (D8), 82 (F10) and 150 (A4) were observed. The major rearrangements of chromosome numbers occured in the first 28 days in culture and did not change significantly between day 28 and day 56. Mouse chromosome #12, which had the largest chromosome fragments in the parent myeloma, remained stable while the number of X chromosomes, which were significantly fragmented already in the parent myeloma, decreased by approximately 50%.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009223102068
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  • 4
    Electronic Resource
    Electronic Resource
    The analysis of oligonucleotide preparations by fractal measures (1998)
    New York : Wiley-Blackwell
    Biopolymers 45 (1998), S. 361-379 
    Details
    ISSN: 0006-3525
    Keywords: fractal measures ; high-performance capillary electrophoresis ; ion-exchange high-performance liquid chromatography ; high-performance liquid chromatography ; oligodeoxyribonucleotides ; oligoribonucleotides ; oligonucleotides ; polymerization processes ; chemical synthesis ; dynamics ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: In this paper we put forward improved mathematical methods for detecting synthesis parameters in connection with analyzing crude products of chemically synthesized oligonucleotides. The crude products experimentally sampled are separated by high-performance capillary electrophoresis and ion-exchange high-performance liquid chromatography. The measured separation profiles of experimental syntheses can be expressed as target and nontarget yields; they are characterized by a few parameters. These parameters account for nonlinear synthesis equations that are solvable by employing iteration procedures. We provide here a theoretical as well as computational analysis based upon specific models for stepwise chain growth.Under nonconstant (nonuniform) conditions we use here an exponential form of growth, with different expressions for calculating the fractal dimension of the biochemical process under study. Step lengths of parameter variations in an interval of finite length have to be adjusted properly to find convergent solutions in a mathematical, regularly four-dimensional parameter space. It is conceivable to have most, if not all, of the calculating and plotting carefully done by a computer.This analysis represents the experimental situation up to 65-mer target oligonucleotides analyzed so far. We thus obtain the dynamics of the polymerization process limited in number by fractal models. The advantage, calculating these new methods as compared to qualitatively judged experimental methods, lies in the satisfactory evaluation of crude products, also of large amounts, of syntheses of these biopolymers. © 1998 John Wiley & Sons, Inc. Biopoly 45: 361-379, 1998
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
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