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  • 1
    Electronic Resource
    Electronic Resource
    Synovial fluid fibronectin fragments: No evidence for a mitogenic effect on fibroblasts (1990)
    Springer
    Rheumatology international 10 (1990), S. 199-201 
    Details
    ISSN: 1437-160X
    Keywords: Rheumatoid arthritis ; Fibronectin ; DNA ; DNA synthesis ; Synovial fluid ; Fibroblasts
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Fragments of bovine plasma fibronectin produced by cathepsin D digestion are reportedly mitogenic for hamster fibroblasts. Rheumatoid arthritis synovial fluid contains many fibronectin fragments, which may contribute to the proliferation of synovial cells. We have therefore investigated the potential of fibronectin fragments to stimulate proliferation of synovial fibroblast-like cells using human material. Affinity-purified human plasma and synovial fluid fibronectin was digested with cathepsin D at pH 3.5 for 0–18 h and proteolysis stopped with pepstatin. A variety of fragments were produced ranging from 50 to 200 kDa when analysed by SDS-PAGE. The proliferative activity of various test preparations was studied using quiescent human skin and synovial fibroblasts. Tests were applied for 24 h to 104 cells and DNA synthesis measured by tritiated thymidine incorporation. Both undigested and peptides of fibronectin consistently failed to stimulate DNA synthesis in fibroblasts at all concentrations tested, compared with a phosphate-buffered saline control. This was in marked contrast to human synovial fluid from either rheumatoid arthritis or osteoarthritis patients, which stimulated DNA synthesis in the same system (P〈0.01). Therefore, our data do not confirm the findings of previous studies in which animal materials were used. We can find no evidence that fibronectin fragments play a role in stimulating synovial proliferation in inflammatory arthritis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02274833
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  • 2
    Electronic Resource
    Electronic Resource
    Effect of oxygen and carbon dioxide on germination and growth ofRhizopus oligosporus on model media and soya beans (1995)
    Springer
    Applied microbiology and biotechnology 43 (1995), S. 908-913 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The microcolony technique enables the effects of several atmospheric conditions on fungal growth to be studied by measuring the radius of the colony, while excluding effects of those conditions on germination of the sporangiospores. Various concentrations of oxygen and carbon dioxide in the gas environment were found to influence growth ofRhizopus oligosporus on malt extract/soya peptone/agar. The maximum radial growth rate was 1.48 mm/h and the maximum specific growth rate was 0.109 h−1 at 30°C. Oxygen became limiting below 1% (v/v), but growth remained possible at levels of 0.001% oxygen. Carbon dioxide stimulated growth at limiting oxygen levels. The specific growth rate increased from 0.043 h−1 at 0.5% (v/v) oxygen and 0% (v/v) carbon dioxide to 0.096 h−1 at 0.5% (v/v) oxygen and 5% (v/v) carbon dioxide. A mixture of 0.5% (v/v) oxygen and 35% (v/v) carbon dioxide inhibited growth. Delay of sporangiospore germination due to low (less than 0.001%) amounts of oxygen was not observed with the techniques used. Fungal activity in a rotating drum fermentor was more strongly affected by low levels of oxygen than was biomass formation on model media. High concentrations of carbon dioxide inhibited growth in the rotating drum fermentor at non-limiting levels of oxygen. It is concluded that aeration and heat removal are both essential aspects of optimization of large-scale solid-substrate bioreactors withRh. oligosporus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02431927
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  • 3
    Electronic Resource
    Electronic Resource
    Effect of oxygen and carbon dioxide on germination and growth of Rhizopus oligosporus on model media and soya beans (1995)
    Springer
    Applied microbiology and biotechnology 43 (1995), S. 908-913 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract  The microcolony technique enables the effects of several atmospheric conditions on fungal growth to be studied by measuring the radius of the colony, while excluding effects of those conditions on germination of the sporangiospores. Various concentrations of oxygen and carbon dioxide in the gas environment were found to influence growth of Rhizopus oligosporus on malt extract/soya peptone/agar. The maximum radial growth rate was 1.48 mm/h and the maximum specific growth rate was 0.109 h-1 at 30 °C. Oxygen became limiting below 1% (v/v), but growth remained possible at levels of 0.001% oxygen. Carbon dioxide stimulated growth at limiting oxygen levels. The specific growth rate increased from 0.043 h-1 at 0.5% (v/v) oxygen and 0% (v/v) carbon dioxide to 0.096 h-1 at 0.5% (v/v) oxygen and 5% (v/v) carbon dioxide. A mixture of 0.5% (v/v) oxygen and 35% (v/v) carbon dioxide inhibited growth. Delay of sporangiospore germination due to low (less than 0.001%) amounts of oxygen was not observed with the techniques used. Fungal activity in a rotating drum fermentor was more strongly affected by low levels of oxygen than was biomass formation on model media. High concentrations of carbon dioxide inhibited growth in the rotating drum fermentor at non-limiting levels of oxygen. It is concluded that aeration and heat removal are both essential aspects of optimization of large-scale solid-substrate bioreactors with Rh. oligosporus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530050504
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Ecological structure of a pelagic seabird community in the Southern Ocean (1982)
    Springer
    Polar biology 1 (1982), S. 39-46 
    Details
    ISSN: 1432-2056
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The pelagic avifauna (excluding penguins) of the African sector (30°S–70°S, 20°W–40°E) of the Southern Ocean is described quantitatively, based on 3,005 10-min observation of seabirds during seven oceanic cruises in April 1979 – April 1980. The avifauna is characterized according to species richness, diversity, abundance and biomass. These indices are correlated with groups of birds ordered into principal diet and feeding-method classes. Birds eating either plankton, cephalopods or a mixed diet accounted for 51, 23 and 22% of the total avifaunal abundance and 22, 49 and 25% of the total biomass, respectively. Piscivores were represented poorly. Planktivores were especially abundant south of the Antarctic Convergence and, to a lesser extent, at the sub-Tropical Convergence. Cephalopod-eaters were most abundant north of the sub-Tropical Convergence. The greatest abundance of omnivores occurred where planktivores and cephalopod-eaters were least abundant. The distribution of the planktivores and cephalopodeaters is related tentatively to the availability of the birds' principal prey.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00568753
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    Paper (German National Licenses)
    Fulltext
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