ISSN:
1420-9071
Keywords:
Glucose dehydrogenase
;
glucose
;
affinity chromatography
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Summary Porcine liver β-d-glucose dehydrogenase, a multi-functional protein, has been purified to apparent homogeneity. The enzyme has been separated from the endoplasmic reticulum using Triton X-114 and further purified using NAD to release glucose dehydrogenase from a NADP-linked sepharose column. The purified enzyme is capable of producing both NADH and NADPH in vivo as indicated by kinetic studies.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01960233
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