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  • 1
    Electronic Resource
    Electronic Resource
    Influence of oxygen transfer rate and media composition on fermentation ofd-xylose byPichia stipitis NRRL Y-7124 (1991)
    Springer
    Journal of industrial microbiology and biotechnology 7 (1991), S. 287-291 
    Details
    ISSN: 1476-5535
    Keywords: Pichia stipitis ; Ethanol productivity ; Oxygen transfer rate ; Media composition ; Xylose
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The optimization of ethanol production byPichia stipitis NRRL Y-7124 was analysed by ATP balance. Ethanol volumetric productivity was maximal (0.5–0.6 g/l h) only over a narrow range of oxygen transfer rates (3–5 mmol O2/l h). Trace element supplements increased ethanol volumetric productivity 20%. Biotin and thiamine did not significantly affect ethanol yield. Vitamins and trace elements were not synergistic. Organic nitrogen source from yeast extract was used for growth simultaneously to ammonia.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01577657
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Spectrophotmetric determination of the settling rate distribution of Pichia stipitis aggregates (1994)
    Springer
    Biotechnology techniques 8 (1994), S. 529-534 
    Details
    ISSN: 1573-6784
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary A method for the determination of the settling rate distribution of yeast aggregates using a spectrophotometer is proposed. The reproducibility of the assay is 5 % in the following conditions: quadruplicate measurements, use of fresh culture medium (pH = 4.7) as diluent, fixed biomass concentration (0.Ds40nm = 1.000), fixed agitation speed (1 cycle/second during 30 seconds). The storage of samples led to a significant loss of the aggregative state. A simulated model was formulated predicting that the scattering range of the distribution would not be affected by the light beam width used or the continuous variation in the absorption coefficient, but a 10 % inaccuracy should be expected for the average distribution estimated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00152140
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    A simple and rapid micro-Kjeldahl method for total nitrogen analysis (1991)
    Springer
    Biotechnology techniques 5 (1991), S. 427-430 
    Details
    ISSN: 1573-6784
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary A fast and simple micro-method for total nitrogen analysis in complex fermentation media is presented. Samples containing up to 50 ug nitrogen are digested in tubes with sodium selenite (in sulphuric-phosphoric mixture) plus hydrogen peroxide during one hour at 380°C. Ammonium liberated is measured without extraction by Berthelot colorimetric reaction.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00155487
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    Paper (German National Licenses)
    Fulltext
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