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  • 1
    ISSN: 1432-0533
    Keywords: Key words Dementia ; DNA fragmentation ; Formalin fixation ; Genomic DNA extraction ; Polymerase chain reaction amplification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We report a method providing access to high molecular weight, polymerase chain reaction (PCR)-amplifiable genomic DNA from brains stored in formalin for many years. It consists mainly of an intensive proteinase K treatment of ground tissue previously embedded in agarose plugs, followed by a washing and an elution step. The method was tested on brains fixed and stored in formalin for up to 46 years. All extracted DNA show an identical pattern of degradation ranging from well-preserved (more than 20 kb) to 400-bp-long fragments. This was demonstrated for DNA extracted from the cerebellums of elderly psychiatric and geriatric patients (of more than 60 years of age), male and female, demented or not, with postmortem delays longer than 1 h and shorter than 1 day. In all these cases PCR amplification of a 838-bp-long β-actin product was successfully performed when proteinase K treatment was sufficiently effective to generate pure DNA. Thus, high molecular weight, PCR-amplifiable genomic DNA can be extracted from brains stored in formalin for almost half a century.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1590-3478
    Keywords: ALL treatment ; CNS side effects ; VEPs
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Somario Sono stati studiati i PVE in pazienti affetti da leucemia acuta linfoblastica per valutare la loro utilità nella diagnosi delle complicanze neurologiche precoci e tardive della terapia combinata chemioantiblastica e radiante di questa malattia. Dai risultati è emerso che: a) è possibile riscontrare, in pazienti esenti da “selflimited somnolence syndrome” e da alterazioni del sistema visivo, un aumento di latenza dei PVE, probabilmente connesso a complicanze neurologiche precoci subcliniche delta irradiazione cranica; b) le complicanze tardive del trattamento chemioantiblastico e radiante (riscontrate attraverso l,esame neurologico di routine, l'EEG e la TA C) sembrano connesse ad un aumento di latenza dei PVE, anche in assenza di alterazioni clinicamente evidenti del sistema visivo.
    Notes: Abstract VEPs were examined in ALL patients in order to test their usefulness in the detection of early and late CNS changes due to combined cranial X-irradiation and chemotherapy. The results suggest that: a) in patients free from self limited somnolence syndrome and visual symptoms, the VEP latency may be increased, probably because of early subclinical CNS changes due to cranial irradiation. b) late CNS changes (detected by routine neurological examination, EEG and CAT) seem to be correlated with increased VEP latency, even in the absence of visual symptoms.
    Type of Medium: Electronic Resource
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