ISSN:
1432-2242
Keywords:
Brassica oleracea
;
Self-incompatibility
;
S-locus
;
Polymerase chain reaction
;
Anther
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary The polymerase chain reaction (PCR) is particularly well suited for the detection of rare sequences. Taking advantage of the recent isolation of sequences associated with stigma self-incompatibility inBrassica oleracea, we used PCR amplifications with primers synthesized to the S6 cDNA sequence, to demonstrate the presence of mRNA homologous to stigmaS-locus gene (SLG) in anthers during early microsporogenesis. In addition, otherS-locus-related (SLR) sequences were shown to be transcribed in sexual as well as in vegetative tissues (roots, leaves), suggesting that the SLG family might be involved not only in pollen-stigma recognition, but more generally in various forms of plant cell signalling processes. This information corroborates the recent discovery of a cDNA-deduced protein kinase from maize roots, whose extracellular receptor displays high homology withBrassica S-locus-specific glycoproteins.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00588600
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