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  • 1
    Electronic Resource
    Electronic Resource
    Short-term cell culture technique for obtaining chromosomes in marine and freshwater fish (1991)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 39 (1991), S. 0 
    Details
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: In this paper we describe a simple and reliable short-term fish cell culture technique, for obtaining chromosome spreads with minimum stress involving the specimens sampled. Fibroblasts were derived from primary cultures from the caudal fin of some economically important freshwater species: northern pike, Esox lucius L., goldfish, Carassius auratus L., and marine species: gilthead sea bream, Sparus aurata L. and cardinal fish, Apogon imberbisL.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1095-8649.1991.tb04411.x
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    The improbability of irradiated spermatozoa as gene transfer vectors in chickens (1990)
    Springer
    Theoretical and applied genetics 80 (1990), S. 228-233 
    Details
    ISSN: 1432-2242
    Keywords: Irradiated sperm ; Transfer vectors ; Chickens
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary It is concluded that chromatin fragments derived from irradiated chicken spermatozoa are not viable vectors for gene transfer. In three experiments conducted at sequential intervals over a period of 1 1/2 years, no marker traits were found in 1,065 G0 progeny from irradiated spermatozoa of Minnesota Dominant Marker males inseminated into recessive Rhode Island Red and White Leghorn females. The inability to secure transformants is ascribed to the following factors: a maximum of five and probably fewer potential vector fragments for each G0 progeny because of irradiation effect on spermatozoan ability to enter the germinal disc; uncertainty of DNA integrity from highly irradiated chromatin; no known mechanism for release of chromatin fragments from irradiated spermatozoa supernumerary pronuclei; and the uncertainty of selective integration into the zygotic nucleus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224391
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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