ISSN:
1432-072X
Keywords:
Myxobacteria
;
Myxobacter AL-1
;
Cell Cycle
;
Selection Synchrony
;
Zonal Centrifugation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Myxobacter AL-1 was synchronized by size fractionation of asynchronous cultures on a 5–20% equivolumetric sucrose gradient in a Ti—15 zonal rotor. The degree of synchronization obtained by this method was determined 1. by a comparison of the size distribution of an asynchronous culture to the distribution of cell numbers in the fractions of the zonal run, 2. by studying the size distributions in the different fractions obtained with the zonal rotor and 3. by following the growth and size distribution of a fraction from a zonal run during one division cycle. The results indicate that this method is suitable for the study of the cell cycle of Myxobacter AL-1.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00446330
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