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  • 1
    Electronic Resource
    Electronic Resource
    Organelle composition in somatic hybrids between an atrazine resistant biotype of Solanum nigrum and Solanum melongena
    Amsterdam : Elsevier
    Plant Science 58 (1988), S. 51-58 
    Details
    ISSN: 0168-9452
    Keywords: Solanum ; atrazine ; isozyme ; protoplast fusion ; somatic hybrid plants
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0168-9452(88)90153-7
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  • 2
    Electronic Resource
    Electronic Resource
    Somatic hybridization between selected Lycopersicon and Solanum species (1991)
    Springer
    Plant cell reports 10 (1991), S. 76-80 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Mesophyll protoplasts of an interspecific Lycopersicon esculentum Mill, (tomato) x Lycopersicon pennellii hybrid plant (EP) were fused with callus-derived protoplasts of Solanum lycopersicoides Dun. using a modified PEG/DMSO procedure. The EP plant was previously transformed by Agrobacterium tumefaciens which carried the NPTII and nopaline synthase genes. Protoplasts were plated at 105/ml in modified KM medium and 16 days post-fusion 25 ug/ml kanamycin was added to the culture medium. During shoot regeneration, 212 morphologically similar putative somatic hybrids were delineated visually from kanamycin resistant EP's. Forty-eight shoots, randomly selected among the 212, were further verified as somatic hybrids by their leaf phosphoglucoisomerase heterodimer isozyme pattern. However, the resulting plants were virtually pollen sterile. In a second fusion, mesophyll protoplasts of Solanum melongena (eggplant) were fused with EP callus-derived protoplasts. Using the same fusion and culture procedure, only two dark green calli were visually selected among the pale green parental EP and verified as somatic cell hybrids by several isozyme patterns. These two calli have produced only leaf primordia in one and half years on regeneration medium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00236461
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  • 3
    Electronic Resource
    Electronic Resource
    Plant regeneration from leaf protoplasts of Solanum torvum (1987)
    Springer
    Plant cell reports 6 (1987), S. 302-304 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A protocol to obtain regenerated plants from protoplasts of Solanum torvum Sw a wild species of eggplant resistant to Verticillium wilt is reported. Leaf protoplasts were enzymatically isolated from six-week old seedlings grown in a controlled environment chamber. Protoplasts were plated on modified KM medium (0.4 M glucose)+(mg/l): 1.0 p-chlorophenoxyacetic acid (CPA)+1.0 naphthaleneacetic acid (NAA)+0.5 6-benzylaminopurine (BAP) and 0.02 abscisic acid (ABA). The protoplast density was 5×104 per ml with 5 ml placed in each of two quadrants in X-dishes (100×15 mm). The reservoir medium was modified KM+(mg/l): 0.1 NAA+0.5 BAP+0.1 M sucrose+0.1 M mannitol+0.6% washed agar+1% activated charcoal. Dishes were initially placed in the dark at 27°C. Protoplast division was initiated in 1–2 weeks and 4 weeks later p-calli were 1–3 mm. Plating efficiency was 11% when measured at 3 weeks. Six-week old p-calli were transferred individually onto Whatman No. 1 filter paper layered on modified KM (0.15 M sucrose)+mg/l: 2.0 indoleacetic acid (IAA)+2.0 zeatin+0.5% washed agar for 2 weeks. Subsequently, shoots occurred within 4 weeks at 70% efficiency on MS+30 g/l sucrose+2 mg/l zeatin. Shoots were rooted on half strength MS+10 g/l sucrose.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00272004
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  • 4
    Electronic Resource
    Electronic Resource
    Interspecific somatic hybrid plants between eggplant (Solanum melongena) and Solanum torvum (1988)
    Springer
    Theoretical and applied genetics 76 (1988), S. 490-496 
    Details
    ISSN: 1432-2242
    Keywords: Cell fusion ; Isozymes ; Solanum melongena ; Solanum torvum ; DNA-DNA hybridization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Mesophyll protoplasts of eggplant (cv Black Beauty) and of Solanum torvum (both 2n=2x=24) were fused using a modification of the Menczel and Wolfe PEG/DMSO procedure. Protoplasts post-fusion were plated at 1 × 105/ml in modified KM medium, which inhibited division of S. torvum protoplasts. One week prior to shoot regeneration, ten individual calluses had a unique light-green background and were verified as cell hybrids by the presence of the dimer isozyme patterns for phosphoglucoisomerase (PGI) and glutamate oxaloacetate transaminase (GOT). Hybridity was also confirmed at the plant stage by DNA-DNA hybridization to a pea 45S ribosomal RNA gene probe. The ten somatic hybrid plants were established in the greenhouse and exhibited intermediate morphological characteristics such as leaf size and shape, flower size, shape, color and plant stature. Their chromosome number ranged from 46–48 (expected 2n=4x=48) and pollen viability was 5%–70%. In vitro shoots taken from the ten hybrid plants exhibited resistance to a verticillium wilt extract. Total DNA from the ten hybrids was restricted and hybridized with a 5.9 kb Oenothera chloroplast cytochrome f gene probe, a 2.4 kb EcoRI clone encoding mitochondrial cytochrome oxidase subunit II from maize and a 22.1 kb Sal I mitochondrial clone from Nicotiana sylvestris. Southern blot hybridization patterns showed that eight of ten somatic hybrids contained the eggplant cpDNA, while two plants contained the cpDNA hybridization patterns of both parents. The mtDNA analysis revealed the presence of novel bands, loss of some specific parental bands and mixture of specific bands from both parents in the restriction hybridization profiles of the hybrids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00260897
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  • 5
    Electronic Resource
    Electronic Resource
    T-DNA-tagged chromosome 12 in donor Lycopersicon esculentum × L. pennellii is retained in asymmetric somatic hybrids with recipient Solanum lycopersicoides (1993)
    Springer
    Theoretical and applied genetics 86 (1993), S. 377-382 
    Details
    ISSN: 1432-2242
    Keywords: Asymmetric hybridization ; Cell fusion ; Lycopersicon esculentum × L. pennellii-Solanum lycopersicoides ; T-DNA tag
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Asymmetric somatic hybrid plants were recovered after fusing irradiated mesophyll protoplasts of donor Lycopersicon esculentum × L. pennellii (EP) interspecific hybrid with callus-derived protoplasts of recipient Solanum lycopersicoides. EP plant A54 had been previously transformed by an agrobacterium vector, and the T-DNA insert mapped to the L. esculentum chromosome 12. The T-DNA insert conferred kanamycin resistance to EP that was subsequently used to select cell fusion products and recover asymmetric hybrid plants that retained tagged chromosome 12. Doses of 50- and 100-Gy irradiation promoted the elimination of only a few donor chromosomes. At 200 Gy, the regenerated plants had ploidy levels higher than tetraploid. However, the T-DNA tagged chromosome 12 was always retained in the asymmetric hybrid plants tested. Likewise, all plants from the 100-Gy series, with the exception of number 160, were mixoploid in the root-tip cells. Such mixoploid asymmetric somatic hybrids could be stabilized by inducing adventitious shoots on leaf strips cultured on shoot regeneration medium containing kanamycin. The asymmetric hybrid plants did not produce viable seed when self-pollinated or backcrossed to tomato or S. lycopersicoides.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222105
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  • 6
    Electronic Resource
    Electronic Resource
    The relationship between a nuclear restorer gene and the nuclear gene for male sterility in Petunia (1984)
    Springer
    Theoretical and applied genetics 67 (1984), S. 475-478 
    Details
    ISSN: 1432-2242
    Keywords: Cytoplasmic male sterility (cms) ; Nuclear male sterility (nms) ; Fertility restorer (Rf) ; Normal cytoplasm-Cytoplasmic male sterility inducing elements (ste)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary This paper describes the relationship between the restorer gene and the gene for male sterility in the background of normal cytoplasm. We combined these two traits by crosses in one plant, thus making genetic analysis possible. Two main conclusions can be drawn: 1. The restorer gene and the gene for male sterility are located at different loci which segregate independently one from the other. 2. The Rf allele does not affect the expression of the e allele.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00263416
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  • 7
    Electronic Resource
    Electronic Resource
    Morphological and molecular characterization of somatic hybrid plants between Lycopersicon esculentum and Solanum nigrum (1988)
    Springer
    Molecular genetics and genomics 212 (1988), S. 191-198 
    Details
    ISSN: 1617-4623
    Keywords: Protoplast fusion ; Polyethylene glycol ; Southern hybridization ; Solanum nigrum ; Lycopersicon esculentum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Mesophyll protoplasts of tomato (Lycopersicon esculentum Mill. var. cerasiforme) and of an atrazine-resistant biotype of black nightshade, (Solanum nigrum L.), were fused by using polyethylene glycol/dimethyl sulfoxide (PEG/DMSO) solution and three somatic hybrid plants, each derived from a separate callus, were recovered. A twostep selection system was used: (1) protoplast culture medium (modified 8E) in which only tomato protoplasts formed calluses; and (2) regeneration medium (MS2Z) on which only S. nigrum calluses produced shoots. These selective steps were augmented by early isozyme analysis of putative hybrid shoots still in vitro. Phosphoglucoisomerase (PGI) and glutamate oxaloacetate transaminase (GOT) mapped to five loci on four chromosomes in tomato confirmed the hybrid nature of the nuclei of regenerated shoots. The somatic hybrid plants had simple leaves, and intermediate flower and bud morphology, but anthesis was reduced to 5% due to premature bud abscission and the pollen grains were non-viable. Southern DNA blot hybridization using a pea 45 S ribosomal RNA gene probe reconfirmed the hybrid nature of the nuclear genome of the three plants. A 32P-labeled probe of Oenothera chloroplast DNA (cpDNA) hybridized to cpDNA restricted with EcoRI or EcoRV indicated the presence of the tomato cpDNA pattern in all three hybrids. Likewise, the plants were all found to be atrazine sensitive. Analysis with two mitochondrial (mt)DNA-specific probes, maize cytochrome oxidase subunit II and PmtSylSa8 from Nicotiana sylvestris, showed that, in addition to typical mitochondrial rearrangements, specific bands of both parents were present or missing in each somatic hybrid plant.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00334683
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