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Notes: The components of human leucocyte dialysate thought to contain transfer factor, which irresponsible for its capacity to augment antigen or mitogen induced lymphocyte blasogenesis in vitro, were determined by comparing the activity of the original dialysate with its fractions derived from chromatography on Sephadex G-10. Augmentation of leucoagglutinin (LA) induced transformation was only observed using the original dialysate and two fractions (IIb and IIc) eluting before the total volume of the column. These fractions contained the majority of free amino acids in the dialysate, and no trichloroacetic acid (TCA) precipitable material. Synthetic mixtures, containing the same amount of free amino acids as the fractions, augmented both tuberculin PPD and LA induced blastogenesis in a similar manner to the fractions. The augmenting activity of the synthetic mixture and hence, presumably, the dialysate fractions seemed to be due mostly to l-serine and glycine, non-essential amino acids not present in MEM-S, the culture medium used. No augmenting affect by leucocyte dialysate was seen when MEM-S was replaced by RPMI 1640, a culture medium containing both l-serine and glycine. The results suggest that the non-specific in vitro activity of leucocyte dialysate in this test may be due to a medium supplementation effect and that this phenomenon is probably not relevant to the nonspecific consequences of injection of transfer factor in vivo.

Notes: In a previous study, and in the present study, we have found that the baseline plasma samples of patients with asthma contain average levels of an endogenous heparin-like material (EHM) that is significantly higher than that noted in non-allergic, non-asthmatic controls. This material appears to have properties of both heparin and heparan sulfate. Three out of six patients responding to inhalational antigen challenge displayed an acute increment in EHM concentration that coincided with a fall in FEV1 values. The relation of EHM concentration to provoked asthma, or to asthma in general, remains to be determined.

Notes: [Auszug] CHALONES have been proposed as diffusible messenger molecules responsible for a negative feedback mechanism controlling cell division. They are, it is claimed, tissue specific but not species specific. Experiments designed to show that tissue extracts contain such inhibitors of cell division have ...

Notes: Summary The distribution of a crop rooting system can be defined by root length density (RD), root length (RL) per soil layer of depth Δz, sum of root length (SRL) in the soil profile (total root length) or rooting depth (z r . The combined influence of these root system parameters on water uptake is not well understood. In the present study, field data are evaluated and an attempt is made to relate a daily “maximum water uptake rate” (WUmax) per unit soil volume as measured in different soil layers of the profile to relevant parameters of the root system. We hypothesize that local uptake rate is at its maximum when neither soil nor root characteristics limit water flow to, and uptake by, roots. Leaf area index and the potential evapotranspiration rate (ET p ) are also important in determining WUmax, since these quantities influence transpiration and hence total crop water uptake rate. Field studies in Germany and in Western Australia showed that WUmax depends on RD. In general, there was a strong correlation between the maximum water uptake rate of a soil layer (LWUmax) normalized by ET p and RL normalized by SRL. The quantity LWUmax · ET p -1 was linearly related to (RL/SRL)1/2. The data show that the single root model will not predict the influence of RD on WUmax correctly under field conditions when water-extracting neighboring roots may cause non-steady-state conditions within the time span of sequential observations. Since the rooting depth z r was linearly related to (SRL)1/2, the relation: LWUmax · ET p -1 = f (RL1/2/z r ) holds. Furthermore it was found that the maximum “specific” uptake rate per cm root length URmax was inversely related to RD1/2 and to SRL1/2 or z r of the profile. Observed high specific uptake rates of shallow rooted crops might be explained not only by their lower RD-values but also by the additional effect of a low z r . The relations found in this paper are helpful for realistically describing the “sink term” of dynamic water uptake models. Growing plants extract water from the soil to meet transpiration needs. Rates of transpiration and of water uptake are set by evaporative demand and by plant and soil factors which influence capacity to meet that demand. These factors include crop canopy size and leaf characteristics, root system characteristics and hydraulic properties of the soil and the soil-root interface. Soil and root system properties vary with depth and all factors vary in time, so that parameters related to them require constant updating over a crop season. Dynamic simulation models describe water uptake by root systems under field conditions as a function of soil depth and time. Many of these simulation approaches are based on Gardner's (1960) single root model (Feddes 1981). These simulation procedures follow the assumption that water uptake is proportional to a difference in water potential between the bulk soil and the root surface or the plant interior, to the hydraulic conductivity of the soil-plant system and to the “effectiveness” of competing roots in water uptake. The effectiveness factor accounts more or less empirically for the influence of various root system parameters on water uptake such as percentage of “active” roots absorbing water, root surface permeability, root length density determining the distance between neighbouring roots, or total root length and depth of the root system. Such models however, will not always reflect correctly the influence of root system characteristics on water uptake since these assumptions have rarely been tested under field conditions. In many instances, there is better agreement between simulated and measured total water use of plants than between predicted and observed water depletion by roots within individual layers of the soil profile (Alaerts et al. 1985). Water uptake by an expanding root system as a function of depth and time has been studied under field conditions for several crops (listed in Herkelrath et al. 1977a; Feddes 1981; Hamblin 1985). They show that the dynamics of water uptake depend on root length density and the “availability” of soil water. However, the combined influence of root length density, total root length and rooting depth on the water uptake pattern has not been assessed. An evaluation of root system parameters with respect to soil water extraction should aid our understanding of how roots perform under field conditions and may assist our efforts to formulate the water uptake function of roots in dynamic simulation studies more realistically. The aim of the present investigation is to develop an approach that relates measured water uptake rates to relevant parameters of the root systems. This approach will be confined to situations where water uptake in a soil layer is not restricted by unfavorable soil conditions, such as in wet soil, by insufficient aeration and, in dry soil, by reduced water flow towards roots or by increased contact resistance (Herkelrath et al. 1977b). We will define a maximum water uptake rate WUmax that is neither soil-limited nor appreciably limited by the decreasing permeability of aging roots. This WUmax will be related to relevant root system parameters as they exist when WUmax is observed. Hence, water uptake by roots in a very wet, as well as in a dry soil, has been excluded from consideration.