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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 8 (1978), S. 0 
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: This is a preliminary report on methods being developed for the isolation of rat AFP messenger RNA and the synthesis of DNA complementary to this mRNA. Using Morris hepatoma 7777 as source material, AFP-synthesizing polysomes have been isolated by indirect immunoprecipitation. The 18S Poly A + RNA (putative mRNAAFP) recovered from these polysomes by differential centrifugation and oligo-dT cellulose chromatography, was translated in a reticulocyte cell-free system into a peptide immunoprecipitable by anti-AFP sera (and not by anti-albumin sera) and co-migrating with native AFP on polyacrylamide gel electrophoresis. DNA complementary to the mRNA was synthesized by the use of avian myeloblastosis virus reverse transcriptase. The cDNA probe is highly homogeneous, as judged by alkaline sucrose gradient sedimentation, and yields a 1/2 C,t value of 8.5 times 10-3 with its template. Preliminary hybridization experiments indicate that sequences hybridizable to the cDNA probe constitute 3.0%, 0.5%, and 0.008% of the total poly A + polysomal RNA populations of 13 day old rat liver, Morris hepatoma 7777 and adult rat liver, respectively.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Clinical and experimental pharmacology and physiology 6 (1979), S. 0 
    ISSN: 1440-1681
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: 1. The biliary excretion of a tracer pulse of sodium [14C] taurocholate injected into the portal vein was compared in adult and near-term foetal sheep.2. Biliary excretion was virtually complete in both adult and foetus, although appreciably faster in the adult.3. These results indicate that at birth the mechanisms for bile salt uptake and excretion in the sheep liver are well established.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Clinical and experimental pharmacology and physiology 21 (1994), S. 0 
    ISSN: 1440-1681
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: 1. Since plasma renin activity is increased in cyclosporin A (CsA)-induced hypertension in the rat, the role of the vascular renin-angiotensin system (RAS) in CsA-induced hypertension was investigated in rat mesenteric resistance vessels.2. Female Wistar rats received CsA (10 mg/kg per day, s.c.) or vehicle for 30 days. CsA treatment increased tail-cuff systolic blood pressure (CsA treated 135 ± 3 mmHg vs control 125 ± 1 mmHg, P〈0.0001).3. Mesenteric resistance arteries (200–300 μm) were isolated and mounted in a microvessel myograph. Concentration-response curves to tetradecapeptide renin substrate (10-11-10−6 mol/L), angiotensin I (10-l1-10−6 mol/L) and angiotensin II (10-12-10−6 mol/L) showed no differences between CsA-treated and control groups.4. Mesenteric vascular angiotensin-converting enzyme (ACE) characteristics were determined by radioligand binding. There were no differences in the content or affinity of ACE between CsA-treated and control rats.5. These results suggest that the mesenteric vascular RAS does not play a major role in CsA-induced hypertension in the rat.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1440-1681
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: 1. The aim of these studies was to determine the role of endothelin in cyclosporin A ([CsA], 10 mg/kg per day, s.c., for 30 days) hypertension in the rat.2. There were no significant differences in plasma endothelin concentrations, in vitro mesenteric vascular membrane [125I]-endothelin-l (ET-1) binding characteristics or myographic vascular responses to endothelin-1 between the CsA and control groups (systolic blood pressure 135 ± 1 vs 127 ± 1 mmHg, respectively, P〈0.001).3. Twenty-four hours after selective endothelin type A receptor blockade in vivo with BQ123, blood pressure in CsA hypertensive rats was lowered to levels of normotensive controls.4. These results suggest that changes in endothelin synthesis rather than changes in vascular sensitivity and/or ET-1 receptor characteristics may contribute to CsA-induced hypertension.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Diabetologia 38 (1995), S. 123-124 
    ISSN: 1432-0428
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Surgical endoscopy and other interventional techniques 6 (1992), S. 179-182 
    ISSN: 1432-2218
    Keywords: Cholelithiasis ; Choledocholithiasis ; Cholecystectomy ; Laparoscopy ; ERCP ; Endoscopic sphincterotomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary After performing selectively 25 laparoscopic cholecystectomies (LC) to determine the place of LC in the management of complicated gallstones, all patients presenting with gallstones were evaluated by the authors for LC. Eighty-six consecutive patients were evaluated and 84 were studied. Follow-up in every case exceeded 6 months. In three of 10 patients with acute cholecystitis, LC was not possible; each had a history longer than 48 h and all had gangrene of the gallbladder. In four patients with empyema, LC was successful, but operative cholangiography failed. Operative cholangiography was successful in 76 of the remaining 77. Of eight patients suspected of having stones in the CBD, cholangiography excluded stones in six and confirmed them in two. Cholangiography identified three other patients with totally unsuspected CBD stones. Of the five patients with CBD stones, four had them flushed to the duodenum at LC following transcystic balloon dilatation of the papilla and one had a post-op. ERCP. Of four patients with acute pancreatitis, three had LC in the same admission. LC was possible in all three patients with morbid obesity. We conclude that with experience, LC is possible for complicated gallstones. In acute cholecystitis, the probability of success is higher with earlier operative intervention. Operative cholangiography is essential. It not only identifies unsuspected CBD stones but also allows LC without ERCP in those with suspected CBD stones and with modification it allows treatment of those stones.
    Type of Medium: Electronic Resource
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