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  • 1
    Electronic Resource
    Electronic Resource
    Monoclonal antibodies against Vibrio salmonicida: the causative agent of coldwater vibriosis (‘Hitra disease’) in Atlantic salmon, Salmo salar L. (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish diseases 11 (1988), S. 0 
    Details
    ISSN: 1365-2761
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Abstract. Monoclonal antibodies (Mab) directed against Vibrio salmonicida were produced and partially characterized. The bacterium is the causative agent of ‘Hitra disease’ or cotdwater vibriosis (CV) and differs from all other Vibrio bacteria tested so far with respect to a unique surface antigen (VS-P1). Thirteen hybridoma clones produced antibodies which exclusively reacted with this antigen in ELISA. The remaining four clones reacted against undefined determinants and were partly cross-reactive to V. anguiilarum, V. ordalii and V. fischeri. Fifteen Mab were of IgG1/kappa and two of the IgG3/kappa isotypes. Eleven of the IgG1 plus the two IgG3 Mab reacted with the VS-P1 molecule.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2761.1988.tb00541.x
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Humoral immune response in Atlantic salmon, Salmo salar L., to cellular and extracellular antigens of Aeromonas salmonicida (1991)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish diseases 14 (1991), S. 0 
    Details
    ISSN: 1365-2761
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Abstract. The putative virulence factors of Aeromonas salmonicida, the aetiological agent of furunculosis in salmonids, are candidates for protective antigens in effective vaeeines against furunculosis. In this report, the authors have compared the immunogenieily of eell-associated and extracellular antigens of A. salmonicida in Atlantic salmon, Salmo salar L., to that in rabbit. The animals were immunized with formalin-killed whole cells and formalin-inactivated extracellular products (ECP), either separately or in combination. The ability of the antigens to induce antibody production was studied by elisa and Western blotting techniques. These results confirm previous reports that far more structures are immunogenie in rabbit compared to the antibody responses elicited in salmon. However, in both species, some antigens were dominant, including a caseinolytic protease in addition to the A-protein and high and low MW LPS.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2761.1991.tb00596.x
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  • 3
    Electronic Resource
    Electronic Resource
    An ultrastructural study of morphological changes in Atlantic salmon, Salmo salar L., during the development of cold water vibriosis (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish diseases 11 (1988), S. 0 
    Details
    ISSN: 1365-2761
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Abstract. The structural changes which occur in the heart, the lateral muscle and the large intestine during the development of cold water vibriosis in Atlantic salmon, Salmo salar L., have been studied by light and electron microscopy. The results strongly suggest a causative relationship between the presence of the fish pathogen, Vibrio salmonicida, and the observed pathological changes. Cell damage was invariably accompanied by the presence of the bacterium. A close relationship was found between the number of bacteria detected in the tissues and the degree of morphological damage. The most severe cell damage was found in areas with a rich blood supply. The earliest sign of damage was detected in the cell coat and cell membrane on the luminal side of the endothelial cells of the capillaries. Later in the development, the bacteria were also found intracellularly in endothelial cells. In moribund fish, the cells in the vicinity of the capillaries were also severely damaged. Bacteria appeared intracellularly in leucocytes even in early stages of the disease. This caused more severe damage to leucocytes compared to erythrocytes, which seldom contained bacteria.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2761.1988.tb00518.x
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  • 4
    Electronic Resource
    Electronic Resource
    A successful vaccination of Atlantic salmon, Salmo salar L., against ‘Hitra disease’ or coldwater vibriosis (1987)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish diseases 10 (1987), S. 0 
    Details
    ISSN: 1365-2761
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Abstract. A Vibrio sp. causing the so-called ‘Hitra disease’ or coldwater vibriosis affecting brood stock Atlantic salmon, Salmo salar L., in Norway, is described by serotyping. Bacteria collected from moribund Atlantic salmon affected by ‘Hitra disease’ in various parts of Norway, were shown to represent a homogeneous group of marine vibrio. A formalized bacterin was made from one isolate. Vaccination of Atlantic salmon parr was carried out by direct immersion in a formalized bacterin prepared from a Vibrio sp. associated with ‘Hitra-disease.’ After 6 weeks the fish were revaccinated and transferred to net pens in the sea. Compared with unvaccinated controls, vaccinated fish demonstrated excellent protection when challenged 6 months later during a natural outbreak of coldwater vibriosis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2761.1987.tb00723.x
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  • 5
    Electronic Resource
    Electronic Resource
    Electrophoretic and immunochemical analysis of surface antigens of the fish pathogens Vibrio salmonicida and Vibrio anguillarum (1990)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish diseases 13 (1990), S. 0 
    Details
    ISSN: 1365-2761
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Abstract. Outer membranes and lipopolysaccharides of the marine fish pathogens Vibrio salmonicida and Vibrio anguillarum were isolated. SDS-PAGE profiles of purified LPS preparations from V. salmonicida revealed a broad low molecular weight band, whereas V. anguillarum LPS profiles demonstrated both a low-molecular band and several weaker high-molecular weight bands. Hydrolysis of V, salmonicida and V. anguillarum LPS separating the polysaccharide chain from the lipid A part and subsequent gel-chromatography suggests a polysaccharide molecular weight of ca. 1000 (‘rough type’ LPS) and ca. 6000 ('smooth type’ LPS), respectively. Western blot of V. salmonicida outer membrane preparations and purified lipopolysaccharides and subsequent immunostaining with mouse monoclonal antibodies was performed. Eleven out of 15 monoclonal antibodies made against V. salmonicida cells reacted with one broad antigen-band in the low molecular weight region of both outer membrane and LPS profiles, corresponding to the LPS region. The previously reported outer surface antigen, VS-P1 from V. salmonicida, was observed to carry LPS epitopes as revealed by binding of monoclonal antibodies to VS-P1 as well as purified LPS preparations. These results strongly suggest that the VS-P1 antigen is a complex of both protein and LPS molecules.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2761.1990.tb00785.x
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    Paper (German National Licenses)
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