ISSN:
1365-3083
Quelle:
Blackwell Publishing Journal Backfiles 1879-2005
Thema:
Medizin
Notizen:
We have characterized the growth-stimulating effect of Interferon-γ (TFN-γ) on various parameters of B cell growth, and compared the effects with those of low molecular weight B cell growth factor (lmw BCGF). We have found that IFN-γ did not affect early changes induced by anti-μ, like initial calcium-flux and rise in mRNA- and protein levels of the proto-oncogene c-myc measured at 3 h. On the other hand, IFN-γ enhanced the effect of anti-μ on parameters measured later in the GI phase of the cell cycle, such as expression of the transferrin receptor and general transcriptional activity, measured as an increase in 7-amitioactinoiTiycin D binding. In particular, whereas the c-myc levels in anti-μ-treated cells peaked al 3 h and then gradually declined. IFN-γ together with anti-μ maintained the c-myc levels at 24 h at approximately the same levels as seen at 3 h. Overall, lmw BCGF had a more potent effect on the parameters affected by IFN-γ, correlating with stronger enhancement of DNA synthesis. However, in contrast to IFN-γ lmw BCGF did not affect anti-μ-induced c-myc mRNA levels. Thus this study has revealed differences between two B cell growth factors in effects on B cell cycle parameters.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1111/j.1365-3083.1991.tb01783.x
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