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  • 1
    Electronic Resource
    Electronic Resource
    Callus formation from cultured protoplasts of banana (Musa sp.)
    Amsterdam : Elsevier
    Plant Science 85 (1992), S. 91-98 
    Details
    ISSN: 0168-9452
    Keywords: Musa sp. ; banana ; callus ; feeder culture ; protoplast
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0168-9452(92)90097-6
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  • 2
    Electronic Resource
    Electronic Resource
    Electrofusion for the production of somatic hybrid plants of Solanum melongena L. and Solanum khasianum C.B. Clark
    Amsterdam : Elsevier
    Plant Science 57 (1988), S. 215-223 
    Details
    ISSN: 0168-9452
    Keywords: S. khasianum ; Solanum melongena ; electrofusion ; protoplast ; somatic hybrids
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0168-9452(88)90127-6
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  • 3
    Electronic Resource
    Electronic Resource
    Somatic hybrid plants produced by electrofusion between Solanum melongena L. and Solanum torvum Sw (1989)
    Springer
    Theoretical and applied genetics 77 (1989), S. 1-6 
    Details
    ISSN: 1432-2242
    Keywords: Solanum melongena ; Solanum torvum ; Protoplasts ; Electrofusion ; Somatic hybrids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Somatic hybrid plants between eggplant (Solanum melongena) and Solanum torvum have been produced by the electrofusion of mesophyll protoplasts in a movable multi-electrode fusion chamber. Using hair structure as a selection criteria, we identified a total of 19 somatic hybrids, which represented an overall average of 15.3% of the 124 regenerated plants obtained in the two fusion experiments. Several morphological traits were intermediate to those of the parents, including trichome density and structure, height, leaf form and inflorescence. Cytological analyses revealed that the chromosome numbers of the somatic hybrids approximated the expected tetraploid level (2n=4x=48). Fifteen hybrid plants were homogeneous and had relatively stable chromosome numbers (46–48), while four other hybrids had variable chromosome numbers (35–48) and exhibited greater morphological variation. The hybridity of these 19 somatic hybrid plants was confirmed by analyses of phosphoglucomutase (Pgm) and esterase zymograms.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00292307
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  • 4
    Electronic Resource
    Electronic Resource
    Plant regeneration from cultured protoplasts of the cooking banana cv. Bluggoe (Musa spp., ABB group) (1993)
    Springer
    Plant cell reports 13 (1993), S. 41-44 
    Details
    ISSN: 1432-203X
    Keywords: Musa spp ; Cooking banana ; Cell suspension ; Somatic embryo ; Protoplast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Suspensions of embryogenic cells of a triploid banana (Musa spp., cv. Bluggoe) were initiated from the uppermost part of meristematic buds, and used as protoplast source. After 20 weeks in culture, the suspension contained a mixture of globular structures or globules and embryogenic cell clusters, as well as single cells. Two types of protoplasts were obtained from embryogenic suspension culture: small (20–30 μm) and larger (30–50 μm) protoplasts with a dense cytoplasm and large starch grains respectively. The small protoplasts probably originated from embryogenic cell clusters, and also from pseudocambial cells of globules, while larger protoplasts were probably released from oval starchy cells and those of the globule peripheral area. In co-culture with a suitable feeder, consisting of suspensions of diploid banana cells, the protoplasts of triploid banana reformed the cell wall within 24 h and underwent sustained divisions leading to the formation of small clusters of 2–3 cells within 7 days. The latter developed directly into embryos without passing through an apparent callus phase. 10% of such embryos gave rise to plantlets when subcultured in 2.2 μM 6-benzylaminopurine and 2 μM 4 amino-3,5,6-trichloropicolinic acid for 1 week, before transfer to MS medium containing 10 μM 6-benzylaminopurine. The rest of the embryos underwent intensive direct secondary embryogenesis which could lead to the formation of plantlets with a frequency of up to 50% upon further transfer to hormone-free medium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232313
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  • 5
    Electronic Resource
    Electronic Resource
    Transformation of potato using mannopine and cucumopine strains of Agrobacterium rhizogenes (1995)
    Springer
    Plant cell, tissue and organ culture 40 (1995), S. 225-230 
    Details
    ISSN: 1573-5044
    Keywords: auxin ; root induction ; Solanum tuberosum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Mannopine and cucumopine strains of Agrobacterium rhizogenes were used for genetic transformation in two cultivars of potato (Solanum tuberosum L.). An overnight pretreatment of stem fragments with NAA prior to bacterial infection was necessary to induce root formation, otherwise very few roots were produced. Whatever the potato cultivar used, rhizogenesis induced by NAA pretreament depended on the bacterial strain. In fact, when explants from both potato cultivars were pretreated with 26.5 μM NAA, on average 84.4% and 71.9% produced roots after inoculation with the strains 2659 and 2659 GUS respectively. On the contrary, few rhizogenic responses (2.0–17.0%) or no response at all (0.0%) were obtained with the strains 15834 and 8196 GUS whatever NAA concentration used. Tests for confirming stable transformation of plant explants by examining both β-glucuronidase activity and the presence of opines showed that 85% of the selected roots were cotransformed. Most of the transformed roots were highly branched and grew rapidly, compared to non-transformed roots with no branching and poor growth. Transgenic plants were readily regenerated with a frequency reaching 80% of total explants tested for both potato cultivars.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00048127
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  • 6
    Electronic Resource
    Electronic Resource
    Direct regeneration of transformed plants from stem fragments of potato inoculated with Agrobacterium rhizogenes (1996)
    Springer
    Plant cell, tissue and organ culture 45 (1996), S. 115-121 
    Details
    ISSN: 1573-5044
    Keywords: cucumopine ; genetic transformation ; shoot regeneration ; Solanum tuberosum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Mannopine and cucumopine strains of Agrobacterium rhizogenes were used for genetic transformation in two cultivars of potato (Solanum tuberisum L.). An overnight pretreatment of internodes with α-naphthaleneacetic acid prior to bacterial infection was found to strongly inhibit shoot formation. On the contrary, infection with bacterial strains enhanced the frequency of shoot formation, compared with the controls, except for the strain 15834 which completely inhibited shoot formation in both potato cultivars. Shoots developed directly from the upper part of both inoculated and control explants, at a frequency ranging from 1 to 5 shoots per fragment. Among 93 shoots regenerated, 9 were found to be opine positive, and exhibited an altered phenotype with shortened internodes. Histological study revealed that the transformed shoots developed directly from cells of the internode sections, and not from induced roots. When grown in an insect-proof tunnel, the transformed plants had both altered and normal phenotypes and were able to produce tubers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00048753
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  • 7
    Electronic Resource
    Electronic Resource
    Plant regeneration in sweet potato (Ipomoea batatas L., Convolvulaceae) (1997)
    Springer
    Euphytica 96 (1997), S. 143-152 
    Details
    ISSN: 1573-5060
    Keywords: Sweet potato ; Ipomoea batatas ; plant regeneration ; somatic embryogenesis ; protoplasts ; genetic variation ; somaclonal variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The application of new techniques for improvement of sweet potato crops, particularly including the exploitation of somaclonal variation, gene transfer by genetic transformation and somatic hybridization, requires the control of plant regeneration from tissue cultures. Shoots can easily be regenerated from explants of stems, petioles, leaves and roots, while callus cultures do not produce any shoots. The potential of somatic embryogenesis and plant regeneration via embryogenesis was evaluated for 10 cultivars of sweet potato. Protocols for plant regeneration from cultured protoplasts have also been developed. Since mesophyll was resistant to enzyme digestion, fragments of stems and petioles, callus and cell suspensions were used as source of protoplasts of sweet potato. Series of transfers of protoplast-derived calluses, particularly those which had been obtained from in vitro plants, to media containing a high level of zeatin resulted in successful formation of shoots in only two sweet potato cultivars. In addition, the embryogenic potential was irreversibly lost through protoplast culture, since protoplasts isolated from embryogenic cell suspensions developed into non-embryogenic callus. Consequently, an alternative protocol is being successfully developed to improve plant regeneration from cultured protoplasts of sweet potato, involving first root formation from which shoots can then be regenerated. Preliminary evaluation in field conditions in Gabon revealed that plants regenerated from cultured protoplasts exhibited a great genetic variability in their growth and tuber formation in particular.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1002997319342
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