ISSN:
1432-072X
Keywords:
Laccase
;
Thioglycolic acid
;
Lignin degradation
;
Polymerization
;
Coniferyl alcohol
;
Catechol
;
Fomes annosus
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract 1. Thioglycolic acid, a Cu-chelating agent, totally inhibited extracellular laccase activity without affecting growth and morphology of Fomes annosus. 2. In the presence of thioglycolic acid Fomes annosus cleaved high molecular weight lignosulfonate with a molecular weight range of 2×106 to 1000. In the absence of thioglycolic acid the polymerizing activity of laccase prevented the detection of lignosulfonate breakdown products. 3. Oxidative polymerization of a lignin monomer, coniferyl alcohol, occurred in the presence but not in the absence of laccase activity. 4. Catechol and guaiacol added to the medium at a concentration of 2 mmol, are normally oxidized by fungal laccase and strongly inhibit growth. Presence of thioglycolic acid prevented the oxidation of these phenols and simultaneously permitted normal growth.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00446882
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