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DNA structure-dependent requirements for yeast RAD genes in gene conversion (1995)

Sugawara, N. ; Ivanov, E. L. ; Fishman-Lobell, J. ; [et al.]

[s.l.] : Nature Publishing Group

Nature 373 (1995), S. 84-86

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ISSN: 1476-4687

Source: Nature Archives 1869 - 2009

Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics

Notes: [Auszug] By physical monitoring of double-strand break (DSB)-induced recombination, the fate of recombining DNA can be observed in real time. A DSB induced at MAT-Z (Fig. 1) is repaired by copying intact mating-type sequences from an unexpressed donor locus, HMLa or HMRa. MAT switching takes one hour to ...

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1038/373084a0

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Double-strand break repair can lead to high frequencies of deletions within short CAG/CTG trinucleotide repeats (1999)

Richard, G.-F. ; Dujon, B. ; Haber, J. E.

Springer

Molecular genetics and genomics 261 (1999), S. 871-882

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ISSN: 1617-4623

Keywords: Key words Trinucleotide repeats ; Double-strand break repair ; Gene conversion ; Yeast

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract Trinucleotide repeats undergo contractions and expansions in humans, leading in some cases to fatal neurological disorders. The mechanism responsible for these large size variations is unknown, but replication-slippage events are often suggested as a possible source of instability. We constructed a genetic screen that allowed us to detect spontaneous expansions/contractions of a short trinucleotide repeat in yeast. We show that deletion of RAD27, a gene involved in the processing of Okazaki fragments, increases the frequency of contractions tenfold. Repair of a chromosomal double-strand break (DSB) using a trinucleotide repeat-containing template induces rearrangements of the repeat with a frequency 60 times higher than the natural rate of instability of the same repeat. Our data suggest that both gene conversion and single-strand annealing are major sources of trinucleotide repeat rearrangements.