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  • 1
    ISSN: 1471-0528
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The association of Chlamydia trachomatis (CT) and herpes simplex virus (HSV) with malignant or premalignant changes in the cervix uteri was studied by determining immunoglobulin A (IgA) antibodies in the cervical secretions of 28 women with inflammatory, 28 with dysplastic, 7 with malignant changes of the uterine cervix, and 26 healthy controls. In cervical secretions IgA antibodies to CT were found in 24 of 35 (69%) patients with malignant or premalignant changes, in 11 of 28 (39%) with cervicitis and in 3 of 26 (12%) controls. IgA antibodies to HSV were found in 10 of 35 (28%) patients with malignant atypia or dysplasia but in none of the women with cervicitis or the controls. The highest frequency of antibodies was found in the patients with cervical carcinoma. Serum IgA antibodies to CT and HSV were found equally in the patients and the controls. Our results suggest that in patients with cervical atypia, local IgA CT antibody production occurs. Whether this association is aetiological or coincidental can not be concluded from this study.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of oral rehabilitation 14 (1987), S. 0 
    ISSN: 1365-2842
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Several theories have been proposed concerning the aetiology of dysfunction of the temporomandibular joint (TMJ). To analyse the relationship of radiographic changes to clinical signs and symptoms of TMJ dysfunction, the present study was conducted in subjects highly predisposed to TMJ involvement, i.e. in patients with rheumatoid arthritis (RA). The aim of the study was to estimate the frequency of disturbances in the masticatory system of RA patients. In addition, the relationship between TMJ abnormalities and the signs and symptoms of dysfunction was investigated.The study consisted of sixty patients with RA and forty control subjects. Asymptomatic subjects were more frequent in the RA group than in the controls. Muscle pain during palpation was recorded in 53.3%, clicking in 53.3%, crepitation in 21.7%, reduced movement capacity of the TMJ in 41.7%, and restricted mouth opening in 31.7% of the RA patients examined. In the control group the most common symptoms and signs were clicking (57.5%), muscle pain during palpation (57.5%) and restricted opening (25.0%).Normal radiographic appearance of the TMJ in RA patients was found in 31.7%. Minor changes comprised 31.7%, moderate changes 21.6%, and severe changes or total loss of the condyle 15.0%. In the control group a normal joint was detected in 87.5%, while minor changes were encountered in 7.5%, and moderate or severe changes only in 5.0%. No relationship was found between Helkimo's anamnestic, clinical or occlusal indices and the severity of the disease graded according the criteria outlined by the American Rheumatism association (ARA). In the ARA group the frequency of signs and symptoms of dysfunction increased with the destructive changes in the TMJ area. In the controls, muscle pain during palpation and clicking sounds in the TMJ were most commonly connected with normal radiographic appearance of the TMJ. In the discriminant analysis the most discriminating factors were crepitation, tenderness to palpation of muscles and movement of TMJ.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] CHEMICALLY defined media designed primarily V_〉t for cell cultures are extensively used for the propagation of viruses in such cultures. To obtain the maximal yield of viruses requiring a lengthy incubation and maturation time in cell cultures, change of the medium must usually be omitted. ...
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Micron And Microscopica Acta 21 (1990), S. 156 
    ISSN: 0739-6260
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Electrical Engineering, Measurement and Control Technology , Natural Sciences in General
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Journal of Chromatography A 397 (1987), S. 321-325 
    ISSN: 0021-9673
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Clinical Biochemistry 16 (1983), S. 361 
    ISSN: 0009-9120
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Clinical Biochemistry 16 (1983), S. 361 
    ISSN: 0009-9120
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1432-1076
    Keywords: Pneumonia ; Respiratory syncytial virus ; Pneumococcus ; Enzyme immunoassay ; Radioimmunoassay
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Viral and bacterial antigen and antibody assays were prospectively applied to study the microbial actiology of community-acquired pneumonia in 195 hospitalised children during a surveillance period of 12 months. A viral infection alone was indicated in 37 (19%), a bacterial infection alone in 30 (15%) and a mixed viral-bacterial infection in 32 (16%) patients. Thus, 46% of the 69 patients with viral infection and 52% of the 62 patients with bacterial infection had a mixed viral and bacterial aetiology. Respiratory syncytial virus (RSV) was identified in 52 patients andStreptococcus pneumoniae in 41 patients. The next common agents in order were non-classifiedHaemophilus influenzae (17 cases), adenoviruses (10 cases) andChlamydia species (8 cases). The diagnosis of an RSV infection was based on detecting viral antigen in nasopharyngeal secretions in 79% of the cases. Pneumococcal infections were in most cases identified by antibody assays; in 39% they were indicated by demonstrating pneumococcal antigen in acute phase serum. An alveolar infiltrate was present in 53 (27%) and an interstitial infiltrate in 108 (55%) of the 195 patients. The remaining 34 patients had probable pneumonia. C-reactive protein (CRP), erythrocyte sedimentation rate and total white blood cell count were elevated in 25%, 40% and 36% of the patients, respectively, CRP was more often elevated in patients with bacterial infection alone than in those with viral or mixed viral-bacterial infections. No other correlation was seen between the radiological or laboratory findings and serologically identified viral, bacterial or mixed viralbacterial infections. By using a comprehensive serological panel, the causative agent could be found in over 50% of patients with pneumonia. We conclude that RSV and pneumococcus are the two most common organisms causing pneumonia in children. Our results suggest that mixed viral-bacterial aetiology is common in lower respiratory tract infections affecting children.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Archives of virology 27 (1969), S. 198-208 
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Hemagglutinin of vesicular stomatitis virus was prepared in suspension culture of BHK21/13 S cells maintained in a medium containing 0.4% bovine albumin and no serum. Optimal conditions for titration of VSV hemagglutinin included a low temperature, pH 5.8 and the use of goose erythrocytes. The hemagglutination pattern, once fully developed, remained fairly stable. Hemagglutinin was completely inactivated in one minute at 56°C; at 35°C it was stable for several days at pH 9.0. In density gradient centrifugation the peak HA activity was found in fractions of 1.22 to 1.24 g/ml, in which virions were also demonstrated by electron microscopy. Nonspecific inhibitors, present in high titers in normal rabbit serum, were removed by modified kaolin treatment. Specific HI titers in hyperimmune rabbit serum were low but increased slightly with ether- or fluoro-carbon-treated hemagglutinins and with a concentrated hemagglutinin.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Archives of virology 91 (1986), S. 93-106 
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Purified measles virions, nucleocapsid protein and crude lysate of measles infected cells were labelled with horseradish peroxidase, and used for the detection of IgM and IgG antibodies to measles virus by direct enzyme immunoassay. The assays consisted of three layers: anti-human IgM or IgG immunoglobulins on solid-phase, test serum specimen and enzyme labelled viral antigen. For the expression of the results, a standard curve was included in each test and the O. D. values were changed to arbitrary antibody units. Specificity and sensitivity of the assays were compared with indirect EIAs. The specificity studies included a collection of serum specimens containing either rheumatoid factor, antinuclear antibodies or IgM antibody specific for other viruses. The assays proved both reliable and simple to perform and sensitivity was slightly higher than that for indirect EIAs. However, specificity was dependent on the purity of the viral antigens. When crude infected cell lysate antigen was used, some nonspecific results were obtained, particularly with serum specimens containing antinuclear antibodies. When virion or nucleocapsid protein were used, no nonspecific reactions were obtained.
    Type of Medium: Electronic Resource
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