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  • 1
    Electronic Resource
    Electronic Resource
    Characterization of a pollen-specific gene from Tradescantia paludosa with an unusual cysteine grouping (1994)
    Springer
    Sexual plant reproduction 7 (1994), S. 201-202 
    Details
    ISSN: 1432-2145
    Keywords: Tradescantia paludosa ; Gene expression ; Microsporogenesis ; Pollen-specific
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00228494
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    PREM-2, a copia-type retroelement in maize is expressed preferentially in early microspores (1996)
    Springer
    Sexual plant reproduction 9 (1996), S. 65-74 
    Details
    ISSN: 1432-2145
    Keywords: Maize ; Retrotransposon ; Microspore expression ; Polygalacturonase genes ; Genome structure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have isolated, by screening a genomic library, a retroelement from maize designated PREM-2 (pollen retroelement maize-2), which is expressed in a tissue-specific manner. RNA transcripts of the PREM-2 family are found in the microspore but not in more mature pollen or in any of the vegetative tissues examined. The expression of PREM-2 elements in the uninucleate microspore provides an explanation for the genetic transmission of genomic rearrangements caused by the transposition of retroelements. PREM-2 elements are very abundant and are estimated to constitute about 5% of the maize genome and could possibly have played an important role in the determination of genome structure and in the generation of repetitive sequences in maize. The entire PREM-2 element is 9439 by long. The LTRs of PREM-2 are 1307 by in length. The internal region between the 5′ and 3′ LTRs contains 6825 by and shares homology to the gag, pro, int, RT, and RNaseH regions of copia-type retroelements. PREM-2 elements have been found in close proximity with several maize genes registered in GenBank. The presence of PREM-2 sequence in the exact 5' flanking position of three polygalacturonase genes expressed in pollen, has been used to examine the evolution of the polygalacturonase multigene family in maize and to estimate the time of the PREM-2 integration event.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02153053
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Dissection of a pollen-specific promoter from maize by transient transformation assays (1992)
    Springer
    Plant molecular biology 18 (1992), S. 211-218 
    Details
    ISSN: 1573-5028
    Keywords: pollen-specific gene expression ; promoter analysis ; transient assays ; Tradescantia ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have previously reported the isolation and characterization of a gene (Zm 13) from Zea mays which shows a pollen-specific pattern of expression. Stably transformed tobacco plants containing a reporter gene linked to portions of the Zm 13 5′ flanking region show correct temporal and spatial expression of the gene. Here we present a more detailed analysis of the 5′ regions responsible for expression in pollen by utilizing a transient expression system. Constructs containing the β-glucuronidase (GUS) gene under the control of various sized fragments of the Zm13 5′ flanking region were introduced into Tradescantia and Zea mays pollen via high-velocity microprojectile bombardment, and monitored both visually and with a fluorescence assay. The results suggest that sequences necessary for expression in pollen are present in a region from −100 to −54, while other sequences which amplify that expression reside between −260 and −100. The replacement of the normal terminator with a portion of the Zm13 3′ region containing the putative polyadenylation signal and site also increased GUS expression. While the −260 to −100 region contains sequences similar to other protein-binding domains reported for plants, the −100 to −54 region appears to contain no significant homology to other known promoter fragments which direct pollen-specific expression. The microprojectile bombardment of Tradescantia pollen appears to be a good test system for assaying maize and possibly other monocot promoter constructs for pollen expression.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00034950
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Isolation and characterization of pollen-specific maize genes with sequence homology to ragweed allergens and pectate lyases (1993)
    Springer
    Plant molecular biology 23 (1993), S. 1061-1065 
    Details
    ISSN: 1573-5028
    Keywords: allergens ; gene expression ; microsporogenesis ; pectate lyase ; pollen ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A cDNA clone (Zm58.1) was isolated by differential screening from a cDNA library made to mature Zea mays pollen, and shown to be pollen-specific by RNA blot analysis. When this partial-length clone was used to probe a genomic library, a similar but distinct pollen-specific genomic clone (68% sequence identity) was isolated (Zm58.2). The putative proteins coded for by these two clones show sequence homology to several flower-expressed gene products from various plant species, including known pollen allergens from short ragweed (Ambrosia artemisiifolia), and to pectate lyases from the plant pathogenic bacteria Erwinia spp. The two genes map to different chromosomes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00021820
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  • 5
    Electronic Resource
    Electronic Resource
    Promoter sequences from a maize pollen-specific gene direct tissue-specific transcription in tobacco (1990)
    Springer
    Molecular genetics and genomics 224 (1990), S. 161-168 
    Details
    ISSN: 1617-4623
    Keywords: Maize ; Pollen development ; Tobacco ; Promoter ; β-Glucuronidase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A set of 5′ promoter deletions from Zmgl3, a genomic clone of a pollen-specific gene of maize, has been transcriptionally fused to a β-glucuronidase (GUS) reporter gene in the binary vector pBI101. Tobacco leaf disks were transformed and mature plants analyzed for GUS activity directed by the Zmg13 promoter constructs. Transgenic plants containing the 375 by Zmg13 sequence from −314 to +61 relative to the transcription start site transcribed GUS RNA and expressed active GUS enzyme in mature pollen but not in leaves. Plants transformed with a 35S CaMV promoter-GUS transcriptional fusion expressed GUS RNA in leaves but not in pollen. Neither GUS RNA or active enzyme could be detected in pollen or leaves from plants containing a 124 by Zmg13-GUS transcriptional fusion missing the putative Zmg13 TATA box. No GUS RNA or enzyme expression was not detected in non-transformed tobacco. RNA and GUS histochemical analysis of the T1 generation confirmed that the temporal expression pattern of Zmg13-GUS transcription in tobacco followed that of the native gene in maize and that the Zmg13 promoter sequences from the maize gene are able correctly to direct genetically stable, tissue-specific gene expression in transgenic tobacco plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00271548
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    A monocot pollen-specific promoter contains separable pollen-specific and quantitative elements (1998)
    Springer
    Plant molecular biology 38 (1998), S. 663-669 
    Details
    ISSN: 1573-5028
    Keywords: maize ; pollen ; pollen-specific promoter ; transgenic plants ; Arabidopsis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The region of the promoter of the pollen-specific maize gene, ZM13, from -119 to -37 was analyzed by a linker-scanning type of substitution mutagenesis and two areas were shown to be responsible for pollen expression: a proximal region delineated by mutations from -84 to -53 that conferred pollen specificity, and an upstream region delineated by a mutation from -107 to -102 (Q-element) that could increase the expression of the proximal region but showed no ability to cause expression in pollen on its own. Replacement of both of these areas with other sequences including the CaMV 35S promoter failed to replace activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006083725102
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    Paper (German National Licenses)
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