ISSN:
1432-198X
Keywords:
Key words Renal development
;
Arginine vasopressin
;
Prostaglandins
;
Collecting duct
;
Microperfusion
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Immature animals have limited ability to concentrate the urine. This is in part the result of end-organ resistance to arginine vasopressin (AVP). To characterize this response, we measured water absorption in microperfused cortical collecting ducts (iCCD) and outer medullary CD (iOMCD) derived from 2- to 12-day-old rabbits. The roles of adenosine 3′,5′-cyclic monophosphate (cAMP) and prostaglandins were investigated. Baseline osmotic water permeability (Lp, 10−7 cm/atm per s) in the iCCD (20.3±2.4) and iOMCD (19.7±5.6) was not different from mature CCD (mCCD) (14.6±3.1). After AVP, Lp in the iCCD (46.7±10.0) was significantly lower than in the mCCD (114.3±21.8). Neither stimulation with cAMP (85.6±51.3) nor inhibition of endogenous prostaglandin production with indomethacin (57.6±29.8) abolished the blunted response to AVP in the iCCD. We conclude that AVP-stimulated water transport in the iCCD is impaired. The disruption in AVP response is, at least in part, localized distal to cAMP, and is not mediated by prostaglandins.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s004670050572
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