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  • 1
    Electronic Resource
    Electronic Resource
    The Effect of Aluminium and Magnesium Sulphate on the Rate of Ferrous Iron Oxidation by 〈i〉Leptospirillum ferriphilum〈/i〉 in Continuous Culture (2007)
    s.l. ; Stafa-Zurich, Switzerland
    Advanced materials research Vol. 20-21 (July 2007), p. 156-159 
    Details
    ISSN: 1662-8985
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: In heap bioleaching the dissolution of gangue minerals from igneous ore materials canlead to the build-up of considerable concentrations of Mg and Al sulphates in the recycled leachsolution. This may interfere with microbial ferrous iron oxidation, which drives the oxidation of thetarget minerals. The kinetics of the oxidation process have been well studied for Leptospirillum andAcidithiobacillus species in tank systems. Although not directly comparable, kinetic parametersderived for tank systems do apply also for heap bioleach conditions. In the present study the effectof solution concentrations of Mg and Al as sulphate at individual concentrations of 0 to 10 g/L andcombined concentrations 0 to 16 g/L each has been investigated in continuous culture usingLeptospirillum ferriphilum. Increasing the concentrations of the salts increasingly depresses the rateof ferrous iron oxidation and also shifts the viable range more and more into the low potentialregion. Al significantly reduces the amount of carbon maintained in the reactor (assumed to becommensurate with biomass), whereas Mg actually enhances it at low concentrations. In both cases,however, the rate is always depressed. The results indicate that heap cultures are likely to performsub-optimally in those operations where build-up of dissolved gangue minerals is not controlled
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/39/transtech_doi~10.4028%252Fwww.scientific.net%252FAMR.20-21.156.pdf
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  • 2
    Electronic Resource
    Electronic Resource
    Nature and significance of oscillatory behavior during solvent production by Clostridium acetobutylicum in continuous culture (1988)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 32 (1988), S. 538-544 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The concurrent production of acids and solvents and the production of acetone during continuous culture in a product-limited chemostat indicated that the culture contained a mixture of acid- and solvent-producing cells. Periodic oscillations in the yield of end products and the specific growth rate of the culture were ob served during undisturbed continuous culture at a constant dilution rate. The increased specific growth rate was associated with an increased acid yield and an increase in the rate of cell division and the proportion of short rods. The decreased specific growth rate was as sociated with an increase in the solvent yield and a decrease in the rate of cell division, resulting in the production of elongated rods. It is proposed that the oscillatory behavior observed during continuous culture is an inherent characteristic related to the shift from primary to secondary metabolism. A major consequence of the oscillation of the specific rates of growth and division in cultures containing acid- and solvent-producing cells is that it precludes the attainment of a true steady state during continuous culture.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260320417
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  • 3
    Electronic Resource
    Electronic Resource
    The effect of micro-aerobic conditions on continuous ethanol production by Saccharomyces cerevisiae (1984)
    Springer
    Biotechnology letters 6 (1984), S. 681-686 
    Details
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The effect of trace amounts of oxygen on the degree of ethanol inhibition in a continuous anaerobic culture of Saccharomyces cerevisiae was studied at the 100 gl −1 feed glucose concentration level. Results showed that the use of micro-aerobic conditions (0,5% of saturation) enhanced the utilisation of substrate by increasing the ethanol tolerance of the yeast without any significant decrease in the ethanol yield per unit substrate consumed. When the results were fitted to an equation of the form % MathType!MTEF!2!1!+-% feaafiart1ev1aaatCvAUfeBSjuyZL2yd9gzLbvyNv2CaerbuLwBLn% hiov2DGi1BTfMBaeXatLxBI9gBaerbd9wDYLwzYbItLDharqqtubsr% 4rNCHbGeaGqiVu0Je9sqqrpepC0xbbL8F4rqqrFfpeea0xe9Lq-Jc9% vqaqpepm0xbba9pwe9Q8fs0-yqaqpepae9pg0FirpepeKkFr0xfr-x% fr-xb9adbaqaaeGaciGaaiaabeqaamaabaabaaGcbaqcLbyacaqG8o% GaaeypaiqabY7agaqcaiaab6cadaWcaaGcbaqcLbyacaqGdbWaaSba% aSqaaKqzagGaae4CaaWcbeaaaOqaaKqzagGaae4qamaaBaaaleaaju% gGbiaabohaaSqabaqcLbyacqGHRaWkcaqGlbWaaSbaaSqaaKqzagGa% ae4CaaWcbeaaaaqcLbyacaGGUaWaaSaaaOqaaKqzagGaae4samaaBa% aaleaajugGbiaabchaaSqabaaakeaajugGbiaabUeadaWgaaWcbaqc% LbyacaqGWbaaleqaaKqzagGaey4kaSIaaeywamaaBaaaleaajugGbi% aabchacaqGZbaaleqaaKqzagGaaiOlaiaacIcacaqGdbWaaSbaaSqa% aKqzagGaae4CaiaabAgaaSqabaqcLbyacqGHsislcaqGdbWaaSbaaS% qaaKqzagGaae4CaaWcbeaajugGbiaacMcaaaaaaa!6301!\[{\text{\mu = \hat \mu }}{\text{.}}\frac{{{\text{C}}_{\text{s}} }}{{{\text{C}}_{\text{s}} + {\text{K}}_{\text{s}} }}.\frac{{{\text{K}}_{\text{p}} }}{{{\text{K}}_{\text{p}} + {\text{Y}}_{{\text{ps}}} .({\text{C}}_{{\text{sf}}} - {\text{C}}_{\text{s}} )}}\]it was found that the values for % MathType!MTEF!2!1!+-% feaafiart1ev1aaatCvAUfeBSjuyZL2yd9gzLbvyNv2CaerbuLwBLn% hiov2DGi1BTfMBaeXatLxBI9gBaerbd9wDYLwzYbItLDharqqtubsr% 4rNCHbGeaGqiVu0Je9sqqrpepC0xbbL8F4rqqrFfpeea0xe9Lq-Jc9% vqaqpepm0xbba9pwe9Q8fs0-yqaqpepae9pg0FirpepeKkFr0xfr-x% fr-xb9adbaqaaeGaciGaaiaabeqaamaabaabaaGcbaGabeiVdyaaja% aaaa!373F!\[{\text{\hat \mu }}\], Ks and Yps were the same as for the non-aerobic case while the ethanol inhibition constant, Kp , had increased from 5,2 to 14,0 gl −1.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00133837
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  • 4
    Electronic Resource
    Electronic Resource
    Ethanol inhibition of continuous anaerobic yeast growth (1982)
    Springer
    Biotechnology letters 4 (1982), S. 39-44 
    Details
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The inhibitory effect of ethanol on the yeast Saccharomyces cerevisiae ATCC 4126 has been studied in continuous culture under conditions where high concentrations of ethanol were produced by the yeast itself. The fermentations were carried out using a glucose, salts medium at glucose concentrations of 20, 100 and 200 gl-1. The growth function $$\mu = \hat \mu \cdot \frac{{C_s }}{{K_s + C_s }} \cdot \frac{{K_p }}{{K_p + Y_{ps} /\left( {C_{sf} - C_s } \right)}}$$ was found to describe the observed data with $$\hat \mu = 1,64h^{ - 1} ,K_s = 3,3g\ell ^{ - 1} $$ $$K_p = 5,2g\ell ^{ - 1} $$ and $$Y_{ps} = 0,43$$ as the values of the kinetic parameters. Data of other workers has also been found to fit the model.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00139280
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