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  • 1
    Electronic Resource
    Electronic Resource
    Characterization of glycosphingolipid mixtures with up to ten sugars by gas chromatography and gas chromatography-mass spectrometry as permethylated oligosaccharides and ceramides released by ceramide glycanase (1989)
    s.l. : American Chemical Society
    Biochemistry 28 (1989), S. 6672-6678 
    Details
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/bi00442a021
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  • 2
    Electronic Resource
    Electronic Resource
    Autoproteolysis coupled to protein folding in the SEA domain of the membrane-bound MUC1 mucin (2006)
    [s.l.] : Nature Publishing Group
    Nature structural & molecular biology 13 (2006), S. 71-76 
    Details
    ISSN: 1545-9985
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] The single cell layer of the lungs and the gastrointestinal tract is protected by the mucus formed by large glycoproteins called mucins. Transmembrane mucins typically contain 110-residue SEA domains located next to the membrane. These domains undergo post-translational cleavage between glycine and ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nsmb1035
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  • 3
    Electronic Resource
    Electronic Resource
    Cystic fibrosis and chloride-secreting diarrhoea (1988)
    [s.l.] : Nature Publishing Group
    Nature 333 (1988), S. 711-711 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] SIR-There must be something wrong with Voorhees and Poggio's image analysis (Nature 333, 364; 1988); their picture of a leopard' is, in fact, of a cheetah. MARK TESTER Department of Botany, University of Cambridge, Cambridge CB2 3EA, UK SIR-In commenting on Rotter and Diamond's article1 on ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/333711c0
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  • 4
    Electronic Resource
    Electronic Resource
    Lack of evidence for an immunosuppressive role for MUC1 (1999)
    Springer
    Cancer immunology immunotherapy 48 (1999), S. 22-28 
    Details
    ISSN: 1432-0851
    Keywords: Key words Tumour antigen ; MUC1 ; T cell ; immunosuppression ; Apoptosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The in vitro anti-proliferative properties of various supernatants from MUC1-expressing cell lines and of purified preparations of MUC1 were evaluated. We have observed that supernatants from the MUC1- and MUC3-positive cell line T47D, but not from the MUC1- and MUC4-positive cell line MCF7, were able to inhibit proliferation of cells from various haematopoietic cell lines. Although the activity of T47D supernatants could be abrogated by immunodepletion of MUC1, immunopurified MUC1 from T47D was unable to inhibit cell proliferation. Significantly, supernatants from mouse 3T3 cells transfected with a secreted form of MUC1 or from BHK-21 cells infected with a recombinant vaccinia virus coding for the secreted form of MUC1, as well as preparations of purified MUC1 from bile or urine, were likewise unable to inhibit T cell proliferation. Surprisingly, a crude mixture of bile mucins had a suppressive effect on T cell growth. Our results suggest that other molecules, such as amino sugars or other mucins, which can associate with MUC1, are likely to be responsible for the observed anti-proliferative effects of T47D cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002620050544
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  • 5
    Electronic Resource
    Electronic Resource
    Lithium evokes a more pronounced natriuresis when administered orally than when given intravenously to salt-depleted rats (1999)
    Springer
    Pflügers Archiv 438 (1999), S. 159-164 
    Details
    ISSN: 1432-2013
    Keywords: Key words Intestine ; Kidney ; Lithium ; Natriuresis ; Sodium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  The effects on renal sodium excretion of giving lithium chloride (LiCl; 0.75 mmol per kg body mass) by gavage or intravenously were investigated. The experiments were carried out on Wistar-Kyoto (WKY) or spontaneously hypertensive (SHR) rats in metabolic cages. The rats had been on a low-salt diet for 4 days. Urine excretion of water, sodium and potassium was followed before and for 24 h after giving LiCl. An oral dose of LiCl evoked a more pronounced renal sodium excretion in either strain of rat as compared to that following intravenous administration, in agreement with previous observations of the effects of giving sodium chloride. Choline chloride (1.5 mmol per kg body mass) given by gavage to WKY rats or SHR evoked no change in the renal excretion of sodium. Based on the results of the present study and on observations reported in the literature, we propose that the intestinal tract contains a sodium ”sensor”, which upon activation releases a natriuretic factor to cause renal sodium excretion. The present results indicate that the proposed ”sensor” is sensitive to lithium but not chloride ions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004240050894
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  • 6
    Electronic Resource
    Electronic Resource
    Molecular characterization of the large heavily glycosylated domain glycopeptide from the rat small intestinal Muc2 mucin (1996)
    Springer
    Glycoconjugate journal 13 (1996), S. 823-831 
    Details
    ISSN: 1573-4986
    Keywords: mucin ; rat small intestine ; sulfated oligosaccharides ; mmunolocalization ; goblet cell ; Muc2
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The largest high-glycosylated domain, glycopeptide A, of the ‘insoluble’ mucin complex of the rat small intestine has earlier been purified and characterized (Carlstedtet al., 1993,J Biol Chem 268: 18771–81). A rabbit antiserum raised against deglycosylated glycopeptide A was used to clone part of a mucin showing homology to the human MUC2 mucin (Hanssonet al., 1994,Biochem Biophys Res Commun 198: 181–90). This serum specifically stained goblet cells (paranuclear) in the mouse small intestine. The size of the coding sequence of glycopeptide A was estimated by using reversed transcriptase PCR of mRNA from an inbred rat strain (GOT-W) using primers in the unique central and C-terminal parts of the proposed rat Muc2 sequences. The PCR and Southern blot of the PCR products showed a fragment of about 5.5 kb corresponding to about 1700 amino acids when the known Cys-rich sequences used for the primers were subtracted. This is slightly larger than the size estimated earlier by biochemical studies. The mRNA encoding the rat Muc2 was slightly smaller than the mRNA encoding the human MUC2 in a colorectal cell line. Although the size of glycopeptide A estimated from biochemical results and by PCR is not identical, the results obtained here further support that the ‘insoluble’ mucin of the rat small intestine is encoded by the Muc2 gene. Most of the oligosaccharides in glycopeptide A were either neutral (40%) or sialylated (40%). The remaining ones were sulfated with the sulfate group attached to C-6 ofN-acetylglucosamine linked to C-6 of theN-acetylgalactosaminitol as revealed by tandem mass spectrometry of the perdeuteroacetylated oligosaccharides. Eighteen oligosaccharides were found of which fourteen were characterized and found to be mostly novel. Our findings thus expand the current knowledge of the core peptide of the rat intestinal goblet cell mucin and provide a relatively complete picture of the glycosylation of a defined mucin domain.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00702346
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  • 7
    Electronic Resource
    Electronic Resource
    Different O-glycosylation of respiratory mucin glycopeptides from a patient with cystic fibrosis (1998)
    Springer
    Glycoconjugate journal 15 (1998), S. 823-833 
    Details
    ISSN: 1573-4986
    Keywords: mucin ; cystic fibrosis ; oligosaccharides ; MALDI ; GC-MS ; CF, cystic fibrosis ; GalNAcol, N-acetylgalactosaminitol ; Hex, hexose ; HexNAc, N-acetylhexosamine ; HexNAcol ; GC, gas chromatography ; MALDI, matrix-assisted laser ; FAB, fast atom bombardment ; MS, mass spectrometry ; CID,collision induced dissociation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The O-linked oligosaccharides from three fractions of highly glycosylated mucin glycopeptides obtained from sputum of a patient with cystic fibrosis were characterized and compared regarding size, composition, sequence and when possible linkage positions. Neutral and sialic acid-containing glycans were permethylated and analyzed by high-temperature GC-MS and MALDI-MS, showing more than 60 different oligosaccharides with a size of up to 15 monosaccharide units. Some of the observed oligosaccharides are novel for respiratory secretions, one being a trifucosylated heptasaccharide with the proposed structure: Fuc-Gal-4(Fuc-3)GlcNAc-(Fuc-)Gal-3GalNAcol. The glycosylation of two of the glycopeptide fractions was similar with regard to the neutral and sialylated oligosaccharides despite their different origins from the sol or gel phase. Analysis of the sulfated oligosaccharides by FAB-MS/MS indicated that the gel fraction contained C-6 linked sulfate groups while the two sol fractions also contained C-3 linked sulfate. The results suggest the presence of different glycosylated mucin domains, probably originating from different mucin glycoforms and/or apoproteins in the airway of cystic fibrosis patients.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006920219069
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  • 8
    Electronic Resource
    Electronic Resource
    Deglycosylation by gaseous hydrogen fluoride of mucus glycoproteins immobilized on nylon membranes and in microtiter wells (1998)
    Springer
    Glycoconjugate journal 15 (1998), S. 749-755 
    Details
    ISSN: 1573-4986
    Keywords: mucin ; glycoprotein ; deglycosylation ; hydrogen fluoride ; trifluoromethanesulfonic acid ; H,F hydrogen fluoride ; TFMSA, trifluoromethanesulfonic acid ; NBT, nitro blue tetrazolium ; BCIP ; BSA, bovine serum albumin ; DELFIA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Strongly reacting antibodies specific for defined mucin gene products are often directed against the mucin protein backbone of the heavily glycosylated serine/threonine rich regions. A prerequisite for the use of such antibodies is often the complete removal of the oligosaccharides from the protein. This paper describes an efficient one-step deglycosylation method using gaseous hydrogen fluoride on nylon blotting membranes and microtiter wells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006935227730
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  • 9
    Electronic Resource
    Electronic Resource
    Developmental changes of blood group A-active glycosphingolipids with type 1 and type 2 chains in rat small intestine (1987)
    Springer
    Glycoconjugate journal 4 (1987), S. 59-71 
    Details
    ISSN: 1573-4986
    Keywords: blood group A-active glycosphingolipids ; type 1 and 2 chains ; development ; rat small intestine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Blood group A-active glycosphingolipids of the small intestine, A-6 and A-12, which have been characterized previously in the adult rat [Breimer ME, Hansson GC, Karlsson K-A, Leffler H (1982) J Biol Chem 257:906–12], were found to appear during postnatal development, using immunostaining on thin layer chromatograms with two monoclonal anti-A antibodies, A005 and A581. In this system, A005 was found to be specific for the A determinant based on the type 2 chain, while A581 reacted mainly with the A determinant based on the type 1 chain and only weakly with the A determinant based on the type 2 chain. A-6 Type 1 was detected first at 18 days after birth. Its concentration increased markedly during the fourth week. A-6 Type 2 was detected, at a very low level, in neonates. Its concentration increased between days 15 and 20 and then decreased almost to the neonate level by 28 days. Dodecaglycosylceramide A-12 followed the same pattern of reactivity as A-6 type 1 with A581, and remained strongly reactive with A005 after 20 days. Linear A-6 and branched A-12 appeared simultaneously. Antibodies directed against blood group H determinants based on the type 1 or type 2 chains did not detect any H structure which might have appeared as a precursor of either A-6 or A-12 at the early stages of postnatal development.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01048445
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  • 10
    Electronic Resource
    Electronic Resource
    Strategy for the investigation of O-linked oligosaccharides from mucins based on the separation into neutral, sialic acid- and sulfate-containing species (1995)
    Springer
    Glycoconjugate journal 12 (1995), S. 69-76 
    Details
    ISSN: 1573-4986
    Keywords: Gas chromatography-mass spectrometry ; oligosaccharide ; mucin ; high performance anion exchange chromatography ; sialic acid ; sulfate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract A method for the separation of O-linked oligosaccharides into neutral, sialic acid-containing and sulfated species was applied to oligosaccharides released by alkaline borohydride from mucin glycopeptides from porcine small intestine. The released mixture of reduced oligosaccharides was applied to an anion exchange column, and the neutral oligosaccharides were collected as the unretarded fraction. A mixture of dimethyl sulfoxide and iodomethane was passed through the column to convert the sialic acid-containing oligosaccharides into methyl esters that were eluted and converted to methyl amides by methyl amine. Finally the sulfated oligosaccharide fraction was eluted with salt. The neutral and the derivatized sialic acid-containing oligosaccharides were analysed by gas chromatography-mass spectrometry after permethylation and the sulfated oligosaccharide fraction was analysed by high performance anion exchange chromatography.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00731871
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