ISSN:
0021-9541
Keywords:
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Medicine
Notes:
Phosphate metabolism in Friend erythroleukemia cells undergoing DMSO-induced differentiation was studied. Thirty minutes after the cells were exposed to DMSO in medium at pH 7, an inhibition of 39% in the incorporation of phosphate into phospholipids was observed. This decrease was not due to a change in the precursor pools since phosphate uptake and the phosphorylation of the organic soluble compounds were only inhibited 13%.Inhibition of phospholipid synthesis preceded inhibition of RNA and protein synthesis and reached a maximum after 24 hours of DMSO treatment. At this time, the phospholipid content of the cells was also decreased as compared to that of the control untreated cells. Phospholipid synthesis remained at a level significantly lower than in the controls over the 4-day observation period, at which time 85% of the treated cells were benzidine positive.Separation of the different phospholipids by chromatography on thin layer silicate gel plates showed that, after one hour of DMSO treatment, more than 80% of the radioactivity was in phosphatidylcholine, phosphatidylserine and phosphatidylethanolamine. Phosphatidylethanolamine was the most inhibited. Incorporation of inositol into phospholipid was also significantly decreased. However, there was little variation in the phospholipid composition of the treated and non-treated cells other than a decrease in the percent of sphingomyelin after 48 hours of DMSO treatment.These changes in phospholipid metabolism may initiate the first step in the complex differentiation process. The phospholipids are important components of membranes and the inducers are known to influence their fluidity.
Additional Material:
2 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jcp.1041010105
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