ISSN:
0741-0581
Keywords:
Beem capsule
;
Monolayer cell growth
;
In situ embedding
;
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Natural Sciences in General
Notes:
A method of growing and embedding monolayer cells in situ on the inner surface of the Beem capsule for light and electron microscopy is described. The results demonstrate that wandering cells in tissue and collected cells in suspension are readily grown on the Beem capsule and embedded in situ by a slight modification of the routine embedding procedure. The method seems to be particularly suited for studies of interactions between growing monolayer cells and various substances, infectious agents, or other types of cells added during the incubation period, where disruption by scraping and pelleting, or enzymatic reaction to remove the cells, would prevent such data from being gathered. The method is also suited for light and electron microscopic studies of minute tissue or organs, such as dissected mosquito salivary glands, whose embedding by the routine procedure is difficult.
Additional Material:
6 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jemt.1060050407
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