ISSN:
1435-702X
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Description / Table of Contents:
Zusammenfassung Bericht über enzymatische Untersuchungen von subretinaler Flüssikeit (SRF) bei Patienten mit primär rhegmatogener Netzhautablösung unterschiedlicher Dauer. Der Proteingehalt in der SRF nahm mit der Dauer der Abhebung zu, ebenso die Aktivitäten der lysosomalen Enzyme (saure Phosphatase, β-Glucuronidase und Kathepsin D). Insbesondere fand sich in der SRF Kathepsin D, das im Serum nicht enthalten war. Die Aktivität eines nicht-lysosomalen Enzyms (Laktat-Dehydrogenase) war die gleiche wie im Serum. Da die lysosomalen Enzyme dafür bekannt sind, daß sie Zellen und Gewebe verändern, trägt die Analyse der Enzyme der SRF zum Verständnis der Pathologie der primär rhegmatogenen Netzhautablösung bei.
Notes:
Summary Samples of subretinal fluid (SRF) from patients with primary rhegmatogeneous retinal detachment of various duration were studied using standard enzymologic techniques. The protein content in SRF increased with the duration of detachment. The activities of lysosomal enzymes in SRF, which were acid phosphatase, β-glucuronidase, and cathepsin D, also increased with the duration of detachment. Especially cathepsin D, which was not detected in serum, was present in SRF. The activity of a nonlysosomal enzyme, i. e., lactic dehydrogenase, which was used as a marker of cell disruption and of serum transudation was the same as that of serum. Because lysosomal enzymes are known to be able to degrade cells and tissues, the enzymologic analysis of SRF contributes to our understanding of the pathology of primary rhegmatogeneous retinal detachment.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00411429
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