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  • 1
    Electronic Resource
    Electronic Resource
    Agrobacterium-mediated transformation of `Alpine' Fragaria vesca, and transmission of transgenes to R1 progeny (1998)
    Springer
    Plant cell reports 17 (1998), S. 279-283 
    Details
    ISSN: 1432-203X
    Keywords: Key words PCR ; Progeny-analysis ; Strawberry ; Transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Agrobacterium-mediated transformation was used to stably introduce β-glucuronidase (gus) and neomycin phosphotransferase (nptII) marker genes into `Alpine' Fragaria vesca FRA 197, a diploid (2n = 2x = 14) strawberry. R0 generation transformants derived from a single clump of kanamycin-resistant callus were vegetatively propagated. The presence of the gus and nptII genes in five clonal R0 runner plants was confirmed by PCR. Southern analysis suggested two sites of nptII insertion. When R1 generation seedlings obtained via self-fertilization of R0 plants were tested by histochemical assay, 591 were GUS positive and 39 were GUS negative. The R1 segregation data fit a 15 : 1 ratio (0.5 〉 P 〉 0.25), consistent with the independent segregation of two transgene insertion loci. These results demonstrate the suitability of `Alpine' F. vesca for transgene research in strawberry.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050392
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Identification of RAPD markers linked to a Phytophthora fragariae resistance gene (Rpf1) in the cultivated strawberry (1997)
    Springer
    Theoretical and applied genetics 94 (1997), S. 1097-1101 
    Details
    ISSN: 1432-2242
    Keywords: Key words Red stele ; Linkage-analysis ; Fragaria×≧≤ananassa ; Bulked-segregant-analysis (BSA) ; Resistance-gene mapping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Bulked segregant analysis (BSA) was used to identify seven random amplified polymorphic DNA (RAPD) markers linked to the Rpf 1 gene. Rpf 1 confers resistance to Phytophthora fragariae var. fragariae, the causal agent of red stele root rot in Fragaria spp. The bulked DNAs represented subsets of a F1 population obtained from the cross Md683×Senga Sengana which consisted of 60 plants and segregated in a 1:1 ratio for resistance or susceptibility to race 2.3.4 isolate NS2 of P.  fragariae. Seven markers were shown to be linked to Rpf 1 and were generated from four primers; five of these markers were in coupling phase and two in repulsion phase with respect to the gene. A linkage map of this resistance gene region was generated using JoinMap 2.0TM. The manner in which Rpf 1 and the linked markers co-segregated indicated that they are inherited in a disomic fashion. These markers could enable gene pyramiding and marker-assisted selection of resistance genes in strawberry breeding programmes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050521
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    Paper (German National Licenses)
    Fulltext
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